Zeng Qinghong, Zhao Yonggang, Yang Yuxian, Chen Xiao Xuan, Wang Geifei, Zhang Peng, Cui Youhong, Su Shaobo, Li Kangsheng
Department of Microbiology and Immunology, The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Guangdong 515041, P.R. China.
Mol Med Rep. 2010 Jul-Aug;3(4):607-11. doi: 10.3892/mmr_00000304.
Cystatin C is a member of the cysteine protease inhibitor family and functions to decrease protease production. A recent study showed that it is aberrantly expressed in many malignant tumors in association with tumor invasion and metastasis. Our study aimed to detect Cystatin C expression in stomach neoplasm tissues and adjacent reparative normal tissues. Samples of cancerous and non-cancerous stomach tissue were obtained via surgery as matched pairs from 12 patients with stomach neoplasms and preserved in paraffin. The expression of Cystatin C in the samples was investigated by immunohistochemistry. Fisher's exact test was used to analyze the relationship between stomach neoplasms and adjacent normal tissues. Additionally, mRNA was extracted and analysed by reverse transcriptase-polymerase chain reaction. The intensity of Cystatin C immunostaining was increased in the tumor tissues compared to the adjacent normal tissues. Cystatin C mRNA expression was increased in stomach neoplasms compared to the normal tissues (p<0.05). The results indicate that the expression of Cystatin C may serve as a marker for stomach neoplasms.
胱抑素C是半胱氨酸蛋白酶抑制剂家族的成员，其作用是减少蛋白酶的产生。最近的一项研究表明，它在许多恶性肿瘤中异常表达，与肿瘤侵袭和转移有关。我们的研究旨在检测胃肿瘤组织和相邻修复正常组织中胱抑素C的表达。从12例胃肿瘤患者手术获取癌性和非癌性胃组织样本作为配对样本，并保存在石蜡中。通过免疫组织化学研究样本中胱抑素C的表达。采用Fisher精确检验分析胃肿瘤与相邻正常组织之间的关系。此外，提取mRNA并通过逆转录-聚合酶链反应进行分析。与相邻正常组织相比，肿瘤组织中胱抑素C免疫染色强度增加。与正常组织相比，胃肿瘤中胱抑素C mRNA表达增加（p<0.05）。结果表明，胱抑素C的表达可能作为胃肿瘤的一个标志物。
