Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg (Saar), Germany.
Anal Bioanal Chem. 2011 Jun;400(7):2093-107. doi: 10.1007/s00216-011-4959-6. Epub 2011 Apr 7.
Multi-analyte procedures are of great interest in clinical and forensic toxicology making the analytical process much simpler, faster, and cheaper and allow monitoring of analytes of different drug classes in one single body sample. The aim of the present study was to validate an ultra high performance liquid chromatographic-tandem mass spectrometric approach for fast target screening and quantification of 34 antidepressants in plasma after simple liquid-liquid extraction as part of a multi-analyte procedure for over 130 drugs. The validation process including recovery, matrix effects, process efficiency, ion suppression/enhancement of co-eluting analytes (already published), selectivity, cross talk, accuracy and precision, stabilities, and limits of quantification and detection showed that the approach was selective, sensitive, accurate, and precise for 28 of the 34 tested drugs. The applicability was successfully tested by analyzing authentic plasma samples and external quality control samples. Furthermore, it could be shown that time- and cost-saving one-point calibration was applicable for 21 drugs for daily routine and especially in emergency cases.
多分析物程序在临床和法医毒理学中非常有意义，使分析过程更加简单、快速和廉价，并允许在单个体样本中同时监测不同药物类别的分析物。本研究的目的是验证一种超高效液相色谱-串联质谱法，用于快速靶向筛选和定量血浆中的 34 种抗抑郁药，这是一种多分析物程序的一部分，用于检测 130 多种药物。验证过程包括回收率、基质效应、过程效率、共洗脱分析物的离子抑制/增强（已发表）、选择性、串扰、准确性和精密度、稳定性以及定量和检测限，结果表明该方法对 34 种测试药物中的 28 种具有选择性、灵敏度、准确性和精密度。通过分析真实的血浆样本和外部质量控制样本成功地测试了适用性。此外，还可以证明，对于 21 种药物，节省时间和成本的单点校准适用于日常工作，特别是在紧急情况下。
