Department of Plant, Soil and Environmental Science, University of Florence, viale delle Idee 30, 50019, Sesto Fiorentino (FI), Italy.
Centro MiRT, Fondazione Minoprio, viale Raimondi 54, 22070, Vertemate con Minoprio (CO), Italy.
Mycorrhiza. 2011 Nov;21(8):703-719. doi: 10.1007/s00572-011-0370-6. Epub 2011 Apr 7.
The aim of this work was to evaluate the effects of selected mycorrhiza obtained in the urban environment on growth, leaf gas exchange, and drought tolerance of containerized plants growing in the nursery. Two-year-old uniform Acer campestre L., Tilia cordata Mill., and Quercus robur L. were inoculated with a mixture of infected roots and mycelium of selected arbuscular (maple, linden) and/or ectomycorrhiza (linden, oak) fungi and grown in well-watered or water shortage conditions. Plant biomass and leaf area were measured 1 and 2 years after inoculation. Leaf gas exchange, chlorophyll fluorescence, and water relations were measured during the first and second growing seasons after inoculation. Our data suggest that the mycelium-based inoculum used in this experiment was able to colonize the roots of the tree species growing in the nursery. Plant biomass was affected by water shortage, but not by inoculation. Leaf area was affected by water regime and, in oak and linden, by inoculation. Leaf gas exchange was affected by inoculation and water stress. V(cmax) and J(max) were increased by inoculation and decreased by water shortage in all species. F(v)/F(m) was also generally higher in inoculated plants than in control. Changes in PSII photochemistry and photosynthesis may be related to the capacity of inoculated plants to maintain less negative leaf water potential under drought conditions. The overall data suggest that inoculated plants were better able to maintain physiological activity during water stress in comparison to non-inoculated plants.
本研究旨在评估城市环境中获得的特定菌根对苗圃中容器化植物生长、叶片气体交换和耐旱性的影响。将 2 年生均匀的欧洲山毛榉、椴树和栎属植物接种混合了受感染根系和选定的丛枝（枫、椴）和/或外生菌根（椴树、栎属）真菌的菌丝体，并在充分浇水或缺水条件下生长。接种后 1 年和 2 年测量植物生物量和叶面积。接种后第一个和第二个生长季节测量叶片气体交换、叶绿素荧光和水分关系。我们的数据表明，本实验中使用的基于菌丝体的接种体能够定殖苗圃中树种的根系。生物量受到缺水的影响，但不受接种的影响。叶面积受到水培制度的影响，在栎属和椴树中，还受到接种的影响。叶片气体交换受到接种和水分胁迫的影响。所有物种中，接种均增加了 V(cmax)和 J(max)，而缺水则降低了 V(cmax)和 J(max)。与对照相比，接种植物的 F(v)/F(m)通常也更高。PSII 光化学和光合作用的变化可能与接种植物在干旱条件下维持较少负叶水势的能力有关。总体数据表明，与未接种植物相比，接种植物在水分胁迫下更能维持生理活性。
