Laboratory of Experimental Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, SP, Brazil.
Vet Res Commun. 2011 Aug;35(6):391-9. doi: 10.1007/s11259-011-9474-x. Epub 2011 Apr 7.
Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction of G0/G1 and G2/M -phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent for the treatment of canine MCT.
肥大细胞瘤（MCT）是犬皮肤和软组织最常见的肿瘤之一。由于肥大细胞瘤表现出多种临床外观和行为，其治疗成为一个挑战。曲古抑菌素 A（TSA）是一种抗真菌抗生素，已显示出对各种类型癌细胞的增殖和诱导凋亡具有抑制作用。为了评估曲古抑菌素 A 作为治疗药物的潜力，培养了 3 级肥大细胞瘤细胞，并以 1 nM 至 400 nM 的 TSA 浓度进行处理。通过 MTT 检测和台盼蓝排斥试验来评估细胞生长和细胞活力，并且评估了细胞周期分析。 TSA 处理显示活细胞数量减少，凋亡诱导的细胞死亡增加。细胞周期分析显示亚二倍体细胞增加，G0/G1 和 G2/M 期减少。根据这些结果，曲古抑菌素 A 可能是治疗犬肥大细胞瘤的一种有前途的化疗药物。
