高效液相色谱-二极管阵列检测法测定工作场所空气中 2,2'-二氯-4,4'-亚甲基二苯胺

A new method for the determination of 2,2'-dichloro-4,4'-methylenedianiline in workplace air samples by HPLC-DAD.

机构信息

Central Institute for Labour Protection-National Research Institute, Warszawa, Poland.

出版信息

Toxicol Mech Methods. 2011 Sep;21(7):554-60. doi: 10.3109/15376516.2011.571726. Epub 2011 Apr 8.

DOI:10.3109/15376516.2011.571726

Abstract

A new procedure has been developed for the assay of 2,2'-dichloro-4,4'-methylenedianiline (MOCA) using high-performance liquid chromatography with diode array detector. MOCA was sampled from workplace air and derivative before determination using 3,5-dinitrobenzoyl chloride. The determination was carried out in the reverse-phase system (mobile phase: acetonitrile: water) using an Ultra C(18) column. The measurement range was 2-40 µg/m(3) for a 100 dm(3) air sample. Limit of detection: 7.9 ng/m(3) and limit of quantification: 23.8 ng/m(3).

摘要

已开发出一种新方法，用于使用带有二极管阵列检测器的高效液相色谱法测定 2,2'-二氯-4,4'-亚甲基二苯胺（MOCA）。使用 3,5-二硝基苯甲酰氯对工作场所空气中的 MOCA 进行采样并衍生后进行测定。测定在反相系统（流动相：乙腈：水）中使用 Ultra C(18)柱进行。测量范围为 100 dm(3)空气样品中 2-40 µg/m(3)。检出限：7.9 ng/m(3)和定量限：23.8 ng/m(3)。