Cao Zubing, Sui Liucai, Li Yunsheng, Ji Suofei, Zhang Xiaorong, Zhang Yunhai
Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei city, Anhui Province 230036, China.
Zygote. 2012 Aug;20(3):229-36. doi: 10.1017/S0967199411000153. Epub 2011 Apr 7.
The present study was to investigate if a completely chemically defined medium (PZM-4) could support the early development of porcine embryos derived from parthenogenetic activation (PA) and cloning (somatic cell nuclear transfer, SCNT), and to lay the foundation for determining the physiological roles of certain supplements in this medium. Porcine embryos derived from PA and SCNT were cultured in media: PZM-3 (a chemically semi-defined medium), PZM-4 (a fully defined medium), and PZM-5 (an undefined medium). Early embryo development was observed. We found that the three medium groups (PZM-3, PZM-4 and PZM-5) exhibited no significant differences in cleavage rates of PA embryos (p > 0.05), while the blastocyst rate in PZM-3 was significantly higher than in PZM-4 and PZM-5 (78.9% vs. 36.0% and 52.3%) (p < 0.05). Moreover, total cell number per blastocyst in PZM-3 was clearly higher than in PZM-5 but similar to that in PZM-4. As for SCNT embryos, no significant differences were observed for the cleavage rates or the blastocyst rates among the three groups (p > 0.05). However, total cell number per blastocyst in PZM-3 was notably higher than in PZM-5, but was similar to that in PZM-4. In conclusion, our results suggested that the completely chemically defined medium PZM-4 can be used to efficiently support the early development of porcine PA and SCNT embryos.
本研究旨在探究完全化学成分明确的培养基（PZM-4）是否能够支持源自孤雌激活（PA）和克隆（体细胞核移植，SCNT）的猪胚胎的早期发育，并为确定该培养基中某些添加物的生理作用奠定基础。将源自PA和SCNT的猪胚胎培养于以下培养基中：PZM-3（化学成分半明确的培养基）、PZM-4（完全化学成分明确的培养基）和PZM-5（化学成分不明确的培养基）。观察早期胚胎发育情况。我们发现，对于PA胚胎，这三种培养基组（PZM-3、PZM-4和PZM-5）的卵裂率无显著差异（p>0.05），而PZM-3中的囊胚率显著高于PZM-4和PZM-5（78.9%对36.0%和52.3%）（p<0.05）。此外，PZM-3中每个囊胚的总细胞数明显高于PZM-5，但与PZM-4中的相似。对于SCNT胚胎，三组之间的卵裂率或囊胚率均未观察到显著差异（p>0.05）。然而，PZM-3中每个囊胚的总细胞数显著高于PZM-5，但与PZM-4中的相似。总之，我们的结果表明，完全化学成分明确的培养基PZM-4可用于有效支持猪PA和SCNT胚胎的早期发育。
