Department of Animal Genetics, Breeding and Reproduction, South China Agricultural University, The New Building of College of Animal Science, Room 315, Guangzhou, 510642, Guangdong, People's Republic of China.
Transgenic Res. 2013 Dec;22(6):1107-18. doi: 10.1007/s11248-013-9729-0. Epub 2013 Jul 16.
The production of animals by somatic cell nuclear transfer (SCNT) is inefficient, with approximately 2% of micromanipulated oocytes going to term and resulting in live births. However, it is the most commonly used method for the generation of cloned transgenic livestock as it facilitates the attainment of transgenic animals once the nuclear donor cells are stably transfected and more importantly as alternatives methods of transgenesis in farm animals have proven even less efficient. Here we describe piggyBac-mediated transposition of a transgene into porcine primary cells and use of these genetically modified cells as nuclear donors for the generation of transgenic pigs by SCNT. Gene transfer by piggyBac transposition serves to provide an alternative approach for the transfection of nuclear donor cells used in SCNT.
体细胞核移植（SCNT）生产动物的效率较低，大约只有 2%的显微操作卵母细胞能发育到足月并最终产下活产仔。然而，它是最常用于克隆转基因家畜的方法，因为一旦核供体细胞稳定转染，就可以获得转基因动物，更重要的是，因为在农场动物中，其他转基因方法已被证明效率更低。在这里，我们描述了 piggyBac 介导的转基因在猪原代细胞中的转位，并使用这些基因修饰细胞作为核供体，通过 SCNT 生成转基因猪。piggyBac 转座子介导的基因转移为 SCNT 中使用的核供体细胞的转染提供了一种替代方法。
