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犬体细胞核移植胚胎在不同培养基中的体外发育

In vitro development of canine somatic cell nuclear transfer embryos in different culture media.

作者信息

Kim Dong-Hoon, No Jin-Gu, Choi Mi-Kyung, Yeom Dong-Hyeon, Kim Dong-Kyo, Yang Byoung-Chul, Yoo Jae Gyu, Kim Min Kyu, Kim Hong-Tea

机构信息

Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration, Suwon 441-706,

出版信息

J Vet Sci. 2015;16(2):233-5. doi: 10.4142/jvs.2015.16.2.233. Epub 2014 Dec 24.

DOI:10.4142/jvs.2015.16.2.233
PMID:25549216
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4483508/
Abstract

The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.

摘要

本研究的目的是调查三种不同培养基对犬体细胞核移植（SCNT）胚胎发育的影响。犬克隆胚胎在改良的合成输卵管液（mSOF）、猪合子培养基-3（PZM-3）或G1/G2序贯培养基中培养。我们的结果表明，与PZM-3（分别为8.5%和0%）或mSOF（2.3%和0%）培养基相比，G1/G2培养基能使犬SCNT胚胎产生显著更高的桑葚胚和囊胚发育率（分别为26.1%和7.8%）。总之，本研究表明，使用G1/G2培养基培养犬SCNT胚胎能够更有效地产生囊胚。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90ab/4483508/78384277f1f0/jvs-16-233-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90ab/4483508/78384277f1f0/jvs-16-233-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90ab/4483508/78384277f1f0/jvs-16-233-g001.jpg

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本文引用的文献

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Theriogenology. 2014 Feb;81(3):419-23. doi: 10.1016/j.theriogenology.2013.10.013. Epub 2013 Oct 18.
2
Effects of DNMT1 and HDAC inhibitors on gene-specific methylation reprogramming during porcine somatic cell nuclear transfer.DNMT1 和组蛋白去乙酰化酶抑制剂对猪体细胞核移植过程中基因特异性甲基化重编程的影响。
PLoS One. 2013 May 31;8(5):e64705. doi: 10.1371/journal.pone.0064705. Print 2013.
3
Effects of chemically defined medium on early development of porcine embryos derived from parthenogenetic activation and cloning.
化学成分明确的培养基对孤雌激活和克隆猪胚胎早期发育的影响。
Zygote. 2012 Aug;20(3):229-36. doi: 10.1017/S0967199411000153. Epub 2011 Apr 7.
4
Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid.克隆牛犊源自于在化学成分明确的培养基或改良的合成输卵管液中培养的体细胞核移植胚胎。
J Vet Sci. 2011 Mar;12(1):83-9. doi: 10.4142/jvs.2011.12.1.83.
5
Effect of mSOF and G1.1/G2.2 media on the developmental competence of SCNT-derived bovine embryos.mSOF和G1.1/G2.2培养基对体细胞核移植牛胚胎发育能力的影响。
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6
In vitro maturation, in vitro fertilization and embryonic development of canine oocytes.
Zygote. 2007 Nov;15(4):347-53. doi: 10.1017/S0967199407004352.
7
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