对韩国九所大学医院进行的血培养进行综合分析。
Comprehensive analysis of blood culture performed at nine university hospitals in Korea.
作者信息
Shin Jeong Hwan, Song Sae Am, Kim Mi-Na, Lee Nam Yong, Kim Eui-Chong, Kim Sunjoo, Koo Sun-Hoi, Ryoo Nam Hee, Kim Jae-Seok, Cho Ji-Hyun
机构信息
Department of Laboratory Medicine, Inje University College of Medicine, Busan, Korea.
出版信息
Korean J Lab Med. 2011 Apr;31(2):101-6. doi: 10.3343/kjlm.2011.31.2.101.
BACKGROUND
Optimal blood culture performance is critical for successful diagnosis and treatment of sepsis. To understand the status of blood culture, we investigated several aspects of the procedure at 9 university hospitals.
METHODS
The process of ordering blood culture sets and sampling volume for adults and children was investigated from January 2010 to April 2010, while the positive rate of detection and growth of skin contaminants were compared in 2009. Microbial growth in aerobic and anaerobic bottles was investigated prospectively.
RESULTS
A majority of the hospitals used 2 sets of bottles for adults and 1 bottle for children. The average blood volume in each set was 7.7 mL for adults and 2.1 mL for children. The positive rate of microorganisms was 8.0%, and the isolation rate of the normal flora of the skin was 2.1%. Bacterial growth rates in aerobic and anaerobic bottles only were 31.8% and 24.5% respectively.
CONCLUSIONS
Ordering blood culture sets and sampling volumes did not comply with CLSI guidelines. However, the rate of positive cultures and skin contamination rates were acceptable. Anaerobic bottles are useful in enhancing the yield of microorganisms.
背景
最佳血培养性能对于脓毒症的成功诊断和治疗至关重要。为了解血培养的现状，我们在9家大学医院对该操作的几个方面进行了调查。
方法
对2010年1月至2010年4月期间成人和儿童血培养瓶的订购流程及采样量进行了调查，同时比较了2009年皮肤污染物的检出率和生长率。对需氧瓶和厌氧瓶中的微生物生长情况进行了前瞻性研究。
结果
大多数医院成人使用2套血培养瓶，儿童使用1套。每套血培养瓶的平均采血量成人是7.7 mL，儿童是2.1 mL。微生物阳性率为8.0%，皮肤正常菌群分离率为2.1%。仅在需氧瓶和厌氧瓶中的细菌生长率分别为31.8%和24.5%。
结论
血培养瓶的订购和采样量不符合CLSI指南。然而，培养阳性率和皮肤污染率是可以接受的。厌氧瓶有助于提高微生物的检出率。
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