Denda K, Konishi J, Hajiro K, Oshima T, Date T, Yoshida M
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
J Biol Chem. 1990 Dec 15;265(35):21509-13.
The nucleotide sequence of the operon of the ATPase complex of an acidothermophilic archaebacterium, Sulfolobus acidocaldarius, has been determined. In addition to the three previously reported genes for the alpha, beta, and c (proteolipid) subunits of the ATPase complex (Denda, K., Konishi, J., Oshima, T., Date, T., and Yoshida, M. (1989) J. Biol. Chem. 264, 7119-7121), the operon contained three other genes encoding hydrophilic proteins with molecular masses 25, 13, and 7 kDa. The 25-kDa protein is the third largest subunit (gamma), the 13-kDa protein is most likely the fourth subunit (delta), and the 7-kDa protein may correspond to an unknown subunit of the ATPase, tentatively named as epsilon subunit. They do not have significant sequence similarity to subunits in F0F1-ATPases and eukaryotic V-type ATPases, whereas the other three subunits, alpha, beta, and c, have homologous counterparts in F0F1- and V-type ATPases. The order of the genes in the operon was delta alpha beta gamma epsilon c. The S. acidocaldarius ATPase operon differed from the eucabacterial F0F1-ATPase operon in that the former contains only one gene for a hydrophobic subunit at the most downstream part of the operon whereas the latter has three hydrophobic F0 genes preceding five hydrophilic F1 genes.
嗜酸嗜热古细菌嗜酸热硫化叶菌（Sulfolobus acidocaldarius）ATP酶复合体操纵子的核苷酸序列已被确定。除了之前报道的ATP酶复合体α、β和c（蛋白脂质）亚基的三个基因外（Denda, K., Konishi, J., Oshima, T., Date, T., and Yoshida, M. (1989) J. Biol. Chem. 264, 7119 - 7121），该操纵子还包含另外三个编码分子量分别为25 kDa、13 kDa和7 kDa亲水性蛋白的基因。25 kDa的蛋白是第三大亚基（γ），13 kDa的蛋白很可能是第四亚基（δ），7 kDa的蛋白可能对应于ATP酶的一个未知亚基，暂命名为ε亚基。它们与F0F1 - ATP酶和真核生物V型ATP酶的亚基没有显著的序列相似性，而另外三个亚基α、β和c在F0F1 - 和V型ATP酶中有同源对应物。操纵子中基因的顺序是δ α β γ ε c。嗜酸热硫化叶菌ATP酶操纵子与真细菌F0F1 - ATP酶操纵子的不同之处在于，前者在操纵子最下游部分仅包含一个疏水亚基的基因，而后者在五个亲水性F1基因之前有三个疏水性F0基因。
