Weeks Andrea, Subbaraman Lakshman N, Jones Lyndon, Sheardown Heather
a School of Biomedical Engineering, McMaster University, Hamilton, ON, Canada L8S 4L7.
J Biomater Sci Polym Ed. 2012;23(8):1021-38. doi: 10.1163/092050611X569060.
The aim of this study was to determine the influence of hyaluronic acid (HA) on lysozyme sorption in model contact lenses containing varying amounts of methacrylic acid (MAA). One model conventional hydrogel (poly(2-hydroxyethyl methacrylate) (pHEMA)) and two model silicone hydrogels (pHEMA, methacryloxypropyltris(trimethylsiloxy)silane (pHEMA TRIS) and N,N-dimethylacrylamide, TRIS (DMAA TRIS)) lens materials were prepared with and without MAA at two different concentrations (1.7 and 5%). HA, along with dendrimers, was loaded into these model contact lens materials and then cross-linked with 1-ethyl-3-(3-dimethylamino propyl)-carbodiimide (EDC). Equilibrium water content (EWC), advancing water contact angle and lysozyme sorption on these lens materials were investigated. In the HA-containing materials, the presence (P < 0.05) and amount (P < 0.05) of MAA increased the EWC of the materials. For most materials, addition of MAA reduced the advancing contact angles (P < 0.05) and for all the materials, the addition of HA further improved hydrophilicity (P < 0.05). For the non-HA containing hydrogels, the presence (P < 0.05) and amount (P < 0.05) of MAA increased lysozyme sorption. The presence of HA decreased lysozyme sorption for all materials (P < 0.05). MAA appears to work synergistically with HA to increase the EWC in addition to improving the hydrophilicity of model pHEMA-based and silicone hydrogel contact lens materials. Hydrogel materials that contain HA have tremendous potential as hydrophilic, protein-resistant contact lens materials.
本研究的目的是确定透明质酸(HA)对含有不同量甲基丙烯酸(MAA)的模型隐形眼镜中溶菌酶吸附的影响。制备了一种传统水凝胶模型(聚甲基丙烯酸2-羟乙酯(pHEMA))和两种硅水凝胶模型(pHEMA、甲基丙烯酰氧基丙基三(三甲基硅氧基)硅烷(pHEMA TRIS)和N,N-二甲基丙烯酰胺、TRIS(DMAA TRIS))镜片材料,有无MAA两种不同浓度(1.7%和5%)。将HA与树枝状聚合物一起加载到这些模型隐形眼镜材料中,然后用1-乙基-3-(3-二甲基氨基丙基)-碳二亚胺(EDC)交联。研究了这些镜片材料的平衡含水量(EWC)、前进水接触角和溶菌酶吸附情况。在含HA的材料中,MAA的存在(P<0.05)和含量(P<0.05)增加了材料的EWC。对于大多数材料,添加MAA降低了前进接触角(P<0.05),对于所有材料,添加HA进一步提高了亲水性(P<0.05)。对于不含HA水凝胶,MAA的存在(P<0.05)和含量(P<0.05)增加了溶菌酶吸附。HA的存在降低了所有材料的溶菌酶吸附(P<0.05)。除了提高基于pHEMA的模型和硅水凝胶隐形眼镜材料的亲水性外,MAA似乎还与HA协同作用,增加EWC。含有HA的水凝胶材料作为亲水性、抗蛋白质隐形眼镜材料具有巨大潜力。