School of Biomedical Engineering, McMaster University, 1280 Main St. West, Hamilton, Ontario.
J Biomed Mater Res A. 2012 Aug;100(8):1972-82. doi: 10.1002/jbm.a.33269. Epub 2012 May 5.
Photocrosslinkable methacrylated hyaluronic acid (HA) was prepared and incorporated into model conventional and silicone hydrogel contact lenses as an internal wetting agent. The molecular weight of the HA, the degree of methacrylation as well as the amount (0.25 to 1.0 wt %) incorporated were varied. The HA-containing hydrogels were analyzed using a variety of techniques including water contact angles, equilibrium water content (EWC), and lysozyme sorption. The presence of HA could be detected in the materials using X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy-attenuated total reflectance. The materials containing methacrylated HA had improved hydrophilicity and reduced lysozyme sorption. Effects of modified HA on EWC were dependent upon the materials but generally increased water uptake. Increased mobility of the HA associated with a lower molecular weight and lower degree of methacrylation was found to be more effective in improving hydrophilicity and decreasing lysozyme sorption than the less mobile HA. All results found suggest that photocrosslinkable HA has significant potential in contact lens applications.
制备了光交联的甲基丙烯酰化透明质酸（HA），并将其作为内部润湿剂掺入模型常规和硅水凝胶隐形眼镜中。HA 的分子量、甲基丙烯酰化程度以及掺入的量（0.25 至 1.0wt%）都有所变化。使用各种技术分析了含有 HA 的水凝胶，包括水接触角、平衡含水量（EWC）和溶菌酶吸附。可以使用 X 射线光电子能谱和傅里叶变换衰减全反射红外光谱检测到材料中的 HA。含有甲基丙烯酰化 HA 的材料具有更好的亲水性和更低的溶菌酶吸附性。修饰后的 HA 对 EWC 的影响取决于材料，但通常会增加水的摄取量。发现与低分子量和低甲基丙烯酰化程度相关的 HA 的高迁移率在改善亲水性和减少溶菌酶吸附方面比低迁移率的 HA 更有效。所有发现的结果都表明，光交联 HA 在隐形眼镜应用中有很大的潜力。
