School of Biomedical Engineering, McMaster University, Hamilton, ON, Canada.
J Biomater Appl. 2012 Nov;27(4):423-32. doi: 10.1177/0885328211410999. Epub 2011 Jul 12.
Model silicone hydrogel contact lenses, comprised of N,N-dimethylacrylamide and methacryloxypropyltris (trimethylsiloxy) silane, were fabricated and hyaluronic acid (HA) was incorporated as an internal wetting agent using a dendrimer-based method. HA and dendrimers were loaded into the silicone hydrogels and cross-linked using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide chemistry. The presence and location of HA in the hydrogels was confirmed using X-ray photoelectron spectroscopy and confocal laser scanning microscopy, respectively. The effects of the presence of HA on the silicone hydrogels on hydrophilicity, swelling behavior, transparency, and lysozyme sorption and denaturation were evaluated. The results showed that HA increased the hydrophilicity and the equilibrium water content of the hydrogels without affecting transparency. HA also significantly decreased the amount of lysozyme sorption (p < 0.002). HA had no effect on lysozyme denaturation in hydrogels containing 0% and 1.7% methacrylic acid (MAA) (by weight) but when the amount of MAA was increased to 5%, the level of lysozyme denaturation was significantly lower compared to control materials. These results suggest that HA has great potential to be used as a wetting agent in silicone hydrogel contact lenses to improve wettability and to decrease lysozyme sorption and denaturation.
采用基于树状大分子的方法，制备了由 N,N-二甲基丙烯酰胺和甲基丙烯酰氧基丙基三（三甲氧基硅基）硅烷组成的模型硅胶水凝胶隐形眼镜，并将透明质酸（HA）作为内部润湿剂掺入其中。HA 和树状大分子被载入硅胶水凝胶中，并使用 1-乙基-3-(3-二甲基氨基丙基）-碳二亚胺化学交联。分别使用 X 射线光电子能谱和共聚焦激光扫描显微镜确认了 HA 在水凝胶中的存在和位置。评估了 HA 对水凝胶亲水性、溶胀行为、透明度、溶菌酶吸附和变性的影响。结果表明，HA 增加了水凝胶的亲水性和平衡水含量，而不影响透明度。HA 还显著降低了溶菌酶的吸附量（p < 0.002）。HA 对含 0%和 1.7%甲基丙烯酸（MAA）（重量）的水凝胶中的溶菌酶变性没有影响，但当 MAA 的量增加到 5%时，与对照材料相比，溶菌酶变性的水平明显降低。这些结果表明，HA 具有很大的潜力作为硅胶水凝胶隐形眼镜的润湿剂，以提高润湿性并减少溶菌酶的吸附和变性。
