School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada.
Eye Contact Lens. 2013 Mar;39(2):179-85. doi: 10.1097/ICL.0b013e318281ae06.
This study was designed to assess the duration of hyaluronic acid (HA) release from model contact lens materials when HA was physically incorporated into the hydrogel during synthesis and to assess the effects of the HA release on lysozyme sorption.
Model conventional and silicone hydrogel contact lens materials containing HA of various molecular weights as a releasable wetting agent were prepared. The HA was released into phosphate-buffered saline and MilliQ water, and the release was monitored using ultraviolet spectroscopy. Hyaluronic acid release was quantified by enzyme-linked immunosorbent assay. The effect of the releasable HA on lysozyme sorption to the materials was also analyzed using 125-I-labeled protein.
Hyaluronic acid loaded into the materials using this method could be released from conventional hydrogel materials for 21 days; the model silicone hydrogels showed release of more than 7 weeks. With one exception, the releasable HA decreased lysozyme sorption.
Hyaluronic acid physically incorporated into contact lens materials during synthesis may therefore be released for extended periods of time of up to 7 weeks. Hyaluronic acid release leads to decreased protein adsorption in general. This method has potential for modification of conventional and silicone hydrogel lenses with releasable HA as a wetting agent.
本研究旨在评估将透明质酸（HA）物理掺入合成过程中的水凝胶中时，模型接触镜材料中 HA 的释放持续时间，并评估 HA 释放对溶菌酶吸附的影响。
制备了含有不同分子量的作为可释放润湿剂的 HA 的模型传统和硅酮水凝胶接触镜材料。将 HA 释放到磷酸盐缓冲盐水和 MilliQ 水中，并使用紫外光谱法监测释放情况。通过酶联免疫吸附试验定量 HA 释放。还使用 125I 标记的蛋白质分析了可释放 HA 对材料中溶菌酶吸附的影响。
用这种方法将 HA 负载到材料中，可以从传统水凝胶材料中释放 21 天；模型硅酮水凝胶的释放时间超过 7 周。除一个例外，可释放的 HA 均降低了溶菌酶的吸附。
因此，在合成过程中物理掺入接触镜材料中的透明质酸可能会在长达 7 周的时间内持续释放。HA 释放通常会导致蛋白质吸附减少。该方法具有用可释放 HA 作为润湿剂修饰传统和硅酮水凝胶镜片的潜力。
