Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia.
Cytokine. 2011 Jul;55(1):104-9. doi: 10.1016/j.cyto.2011.03.012. Epub 2011 Apr 7.
The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling cascade has been identified as a crucial factor for myogenesis. The STAT3 isoform is essential for satellite cell migration and myogenic differentiation as it mediates the expression of muscle specific myogenic factors. The SOCS (suppressors of cytokine signaling) family of proteins down-regulates STAT activation. Primary human skeletal muscle cells were isolated and cultured to investigate the effect of SOCS3 adenoviral overexpression on myotube maturation. It was demonstrated that STAT3 inhibition did not influence myotube development or survival. Moreover, SOCS3 overexpression enhances the mRNA expression of downstream targets of STAT3, c-FOS and VEGF. These increases were correlated with enhanced mRNA expression of genes associated with muscle maturation and hypertrophy. Thus SOCS3 influences myoblast differentiation and SOCS3 may be significant in regulating the activity of genes previously identified as transcriptionally regulated by STAT3.
Janus 激酶/信号转导和转录激活因子（JAK/STAT）信号级联已被确定为肌肉发生的关键因素。STAT3 同工型对于卫星细胞迁移和肌生成分化至关重要，因为它介导肌肉特异性肌生成因子的表达。SOCS（细胞因子信号转导抑制物）家族蛋白下调 STAT 激活。分离并培养原代人骨骼肌细胞，以研究 SOCS3 腺病毒过表达对肌管成熟的影响。结果表明，STAT3 抑制并不影响肌管的发育或存活。此外，SOCS3 过表达增强了 STAT3 下游靶基因 c-FOS 和 VEGF 的 mRNA 表达。这些增加与与肌肉成熟和肥大相关的基因的 mRNA 表达增强相关。因此，SOCS3 影响成肌细胞的分化，SOCS3 可能在调节先前鉴定为 STAT3 转录调控的基因的活性方面具有重要意义。
