Boston University, Department of Biomedical Engineering, Boston, Massachusetts, USA.
Nat Methods. 2011 May;8(5):401-3. doi: 10.1038/nmeth.1595. Epub 2011 Apr 10.
A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count, one-dimensional imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using six-pixel one-dimensional images, we investigated protein localization in a yeast model for human protein misfolding diseases and demonstrated the feasibility of a nuclear-translocation assay in Chinese hamster ovary (CHO) cells expressing an NFκB-EGFP reporter.
一种平行微流控细胞仪 (PMC) 使用高速扫描光电倍增管为基础的探测器将低像素计数、一维成像与流式细胞术相结合。PMC 的 384 个平行流道将计数率与信噪比解耦。使用六像素的一维图像，我们研究了酵母模型中人蛋白错误折叠疾病的蛋白质定位，并证明了核易位测定法在表达 NFκB-EGFP 报告基因的中国仓鼠卵巢 (CHO) 细胞中的可行性。
