Wang Hsiang-Yu, Bao Ning, Lu Chang
School of Chemical Engineering, Purdue University, West Lafayette, IN 47907, USA.
Biosens Bioelectron. 2008 Dec 1;24(4):613-7. doi: 10.1016/j.bios.2008.06.005. Epub 2008 Jun 12.
Microfluidic arrays of living cells have raised a lot of interests recently due to their potential for high throughput screening of cell-based assays. This report presents a microfluidic cell array with individually addressable chambers controlled by pneumatic valves for cell culture and treatment. There are two modes for the cell array to be operated. In the first mode, different groups of cells are directed into designated chambers for culturing and observation. We demonstrate the delivery and culture of enhanced green fluorescent protein (EGFP) expressing and nonfluorescent Chinese hamster ovary (CHO) cells into specific chambers in the array. In the second mode, the chambers are first seeded with the same cell type and different reagents are delivered to specific chambers for cell treatment. We treat cells in designated chambers with Calcein AM and CellTrace calcein red-orange AM to demonstrate the principle. We envision that this microfluidic cell array technology will pave the way to automated high-throughput screening of biomolecules and drugs based on observing cellular phenotypes and responses.
近年来，活细胞微流控阵列因其在基于细胞的检测高通量筛选方面的潜力而引起了广泛关注。本报告展示了一种微流控细胞阵列，其具有由气动阀控制的可单独寻址的腔室，用于细胞培养和处理。该细胞阵列有两种操作模式。在第一种模式下，不同组的细胞被引导至指定腔室进行培养和观察。我们展示了将表达增强型绿色荧光蛋白（EGFP）的细胞和非荧光中国仓鼠卵巢（CHO）细胞输送到阵列中的特定腔室并进行培养。在第二种模式下，先在腔室中接种相同类型的细胞，然后将不同试剂输送到特定腔室进行细胞处理。我们用钙黄绿素AM和CellTrace钙黄绿素红橙AM处理指定腔室中的细胞以证明该原理。我们设想这种微流控细胞阵列技术将为基于观察细胞表型和反应的生物分子和药物自动化高通量筛选铺平道路。
