迁移刺激因子作为唾液腺肿瘤的一种新型生物标志物。

Migration-stimulating factor as a novel biomarker in salivary gland tumours.

机构信息

Unit of Cell and Molecular Biology, Dental School, University of Dundee, UK.

出版信息

J Oral Pathol Med. 2011 Nov;40(10):747-54. doi: 10.1111/j.1600-0714.2011.01044.x. Epub 2011 Apr 11.

DOI:10.1111/j.1600-0714.2011.01044.x

PMID:21481007

Abstract

BACKGROUND

The identification of novel stratification biomarkers would benefit the clinical management of patients with salivary gland tumours. Migration-stimulating factor (MSF) is a potent stimulator of cell invasion, matrix remodelling and angiogenesis. The aim of this study was to determine whether MSF was expressed in salivary gland tumours and its potential value as a diagnostic biomarker.

METHODS

Paraffin-embedded archival specimens of small salivary gland tumours were stained with an MSF-specific antibody. The specimens included 27 malignant and seven benign tumours; histologically normal salivary gland adjacent to the tumour was present in 16 specimens. MSF expression was assessed by consensus of 2-4 independent observers according to various indices, including 'overall MSF grade', 'percentage of area stained' and 'intensity of the staining'. The motogenic effect of MSF on a salivary gland tumour cell line, HSG, was examined in the transmembrane assay.

RESULTS

Overall MSF expression increased significantly in a step-wise fashion from normal salivary gland to benign and malignant tumours (P = 0.04-0.0001); with moderate/strong positive specimens representing 6%, 33% and 74% of the normal, benign and malignant specimens, respectively. MSF was heterogeneously expressed in both carcinoma and stromal cell compartments, its expression being higher in malignant than benign tumours regarding various MSF indices. In tissue culture studies, exogenous MSF stimulated the migration of HSG cells.

CONCLUSIONS

These immunohistochemical and functional studies suggest that MSF expression is a potentially useful biomarker of salivary gland tumour progression.

摘要

背景

鉴定新的分层生物标志物将有益于唾液腺癌患者的临床管理。迁移刺激因子（MSF）是细胞侵袭、基质重塑和血管生成的有效刺激物。本研究旨在确定 MSF 是否在唾液腺癌中表达，及其作为诊断生物标志物的潜在价值。

方法

使用 MSF 特异性抗体对小唾液腺肿瘤的石蜡包埋存档标本进行染色。标本包括 27 例恶性肿瘤和 7 例良性肿瘤；16 例标本中存在肿瘤旁组织学正常的唾液腺。根据“总体 MSF 分级”、“染色面积百分比”和“染色强度”等各种指标，由 2-4 位独立观察者进行共识评估，以评估 MSF 表达。通过跨膜测定法，研究了 MSF 对唾液腺癌细胞系 HSG 的促迁移作用。

结果

从正常唾液腺到良性和恶性肿瘤，MSF 的总体表达呈逐步显著增加（P=0.04-0.0001）；中度/强阳性标本分别占正常、良性和恶性标本的 6%、33%和 74%。MSF 在癌和间质细胞区室中呈异质性表达，在各种 MSF 指标方面，其在恶性肿瘤中的表达高于良性肿瘤。在组织培养研究中，外源性 MSF 刺激了 HSG 细胞的迁移。