大鼠子宫内膜异位症模型中一氧化氮和不对称二甲基精氨酸的水平。

The levels of nitric oxide and asymmetric dimethylarginine in the rat endometriosis model.

作者信息

Cayci Tuncer, Akgul Emin Ozgur, Kurt Yasemin Gulcan, Ceyhan Temel S, Aydin Ibrahim, Onguru Onder, Yaman Halil, Cakir Erdinc, Yasar Mehmet, Bilgi Cumhur, Erbil Kemal M

机构信息

Department of Medical Biochemistry, Gulhane Military Medical Academy, Ankara, Turkey.

出版信息

J Obstet Gynaecol Res. 2011 Aug;37(8):1041-7. doi: 10.1111/j.1447-0756.2010.01482.x. Epub 2011 Apr 12.

DOI:10.1111/j.1447-0756.2010.01482.x

PMID:21481092

Abstract

AIM

To investigate the levels of nitric oxide (NO) and asymmetric dimethylarginine (ADMA) in all the rat endometriosis models.

MATERIAL & METHODS: Forty-one rats with endometriotic implants were divided into four groups (1 to 4) and administered infliximab, etanercept, letrozole and control, respectively. There were 11 rats in group 5 (normal). The size of implants, plasma ADMA and nitrate/nitrite (NO(x) ) levels and histological score were assessed.

RESULTS

In groups 1, 2 and 3, plasma ADMA levels were higher than groups 4 and 5, 296.8 ± 66.2, 285.9 ± 35.7, 200.3 ± 41.0, 125.3 ± 16.7, 111.3 ± 6.5 µmol/L, respectively, while NO(x) levels were lower than groups of control and normal 19.6 ± 3.8, 19.8 ± 4.4, 39.3 ± 6.1, 80.5 ± 5.3, and 91.1 ± 5.0 µmol/L, respectively.

CONCLUSIONS

Infliximab, etanercept and letrozole have regressed endometriotic implants, decreased plasma NO(x) levels, and increased plasma ADMA levels.

摘要

目的

研究所有大鼠子宫内膜异位症模型中一氧化氮（NO）和不对称二甲基精氨酸（ADMA）的水平。

材料与方法

将41只患有子宫内膜异位植入物的大鼠分为四组（1至4组），分别给予英夫利昔单抗、依那西普、来曲唑和作为对照。第5组有11只大鼠（正常）。评估植入物大小、血浆ADMA和硝酸盐/亚硝酸盐（NO(x)）水平以及组织学评分。

结果

在第1、2和3组中，血浆ADMA水平高于第4组和第5组，分别为296.8±66.2、285.9±35.7、200.3±41.0、125.3±16.7、111.3±6.5μmol/L，而NO(x)水平低于对照组和正常组，分别为19.6±3.8、19.8±4.4、39.3±6.1、80.5±5.3和91.1±5.0μmol/L。