van Loveren C, Lammens A J, ten Cate J M
Department of Cariology and Endodontology, Academic Centre for Dentistry Amsterdam, The Netherlands.
Caries Res. 1990;24(5):337-43. doi: 10.1159/000261293.
Fluoride-resistant strains of mutans streptococci have been found to be less cariogenic than their parent strains in the rat model system. Therefore, it is important to determine whether the use of fluorides in vivo will increase the fluoride resistance of mutans streptococci and thereby possibly decrease their cariogenic potential. We studied (1) the fluoride resistance of Streptococcus mutans C180-2 in rat dental plaque exposed to various fluoride treatments (experiment 1), (2) the cariogenic potential of the exposed plaque after transmission to other animals (experiment 2), and (3) whether the fluoride treatments favoured the establishment of in vitro induced fluoride-resistant S. mutans in competition with a fluoride-sensitive strain in rat dental plaque (experiment 3). In all experiments, specific pathogen-free Osborne-Mendel rats were used, and all animals received diet SSP 20/5, containing 20% (w/w) sucrose and 5% (w/w) glucose, and tap water ad libitum. In experiment 1, the rats were inoculated with S. mutans C180-2. For an experimental period of 44 days, the rats either received no F treatment or received NaF mixed in the diet and drinking water (20 or 60 ppm F) or were brushed 6 days per week with an aqueous NaF solution containing either 0.1% (w/v) or 1% (w/v) F, respectively. Bacteriological analyses at the end of the experiment revealed that none of the fluoride treatments had caused a detectable increase in the inherent fluoride resistance of S. mutans C180-2.(ABSTRACT TRUNCATED AT 250 WORDS)
在大鼠模型系统中，已发现变形链球菌的耐氟菌株比其亲本菌株的致龋性更低。因此，确定体内使用氟化物是否会增加变形链球菌的耐氟性，从而可能降低其致龋潜力非常重要。我们研究了：（1）在接受各种氟化物处理的大鼠牙菌斑中变形链球菌C180 - 2的耐氟性（实验1）；（2）将暴露后的菌斑接种到其他动物后其致龋潜力（实验2）；以及（3）氟化物处理是否有利于体外诱导的耐氟变形链球菌在大鼠牙菌斑中与氟敏感菌株竞争中定植（实验3）。在所有实验中，均使用无特定病原体的奥斯本-孟德尔大鼠，所有动物均自由摄取含20%（w/w）蔗糖和5%（w/w）葡萄糖的SSP 20/5饮食及自来水。在实验1中，给大鼠接种变形链球菌C180 - 2。在44天的实验期内，大鼠要么不接受氟处理，要么在饮食和饮用水中添加氟化钠（20或60 ppm氟），要么分别每周用含0.1%（w/v）或1%（w/v）氟的氟化钠水溶液刷牙6天。实验结束时的细菌学分析表明，没有一种氟化物处理能使变形链球菌C180 - 2的固有耐氟性出现可检测到的增加。（摘要截短于250字）
