利用 FITC 荧光检测卵黄蛋白原掺入斑马鱼卵母细胞。
Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence.
机构信息
Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan.
出版信息
Reprod Biol Endocrinol. 2011 Apr 11;9:45. doi: 10.1186/1477-7827-9-45.
BACKGROUND
Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.
METHODS
Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.
RESULTS
Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.
CONCLUSION
This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.
背景
从水泡眼金鱼的眼囊中可以收集到大量的淋巴液。我们试图在水泡眼金鱼的眼囊中诱导卵黄蛋白原（Vtg）的产生，并利用 FITC 标记开发一种可视化斑马鱼卵母细胞中 Vtg 掺入的方法。
方法
雌激素能有效地诱导金鱼眼囊中 Vtg 的产生。FITC 标记的 Vtg 制备后，将其注入成熟的雌性斑马鱼体内。
结果
在体内和体外实验中均检测到 FITC 标记的 Vtg 被斑马鱼卵母细胞所掺入。用 FITC 标记的 Vtg 注射的斑马鱼雌鱼所产生的胚胎，其卵黄囊发出 FITC 荧光，并能正常发育。
结论
这种使斑马鱼卵母细胞掺入 Vtg 的方法可能对斑马鱼卵母细胞的发育和内分泌学实验有用。
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