Microbial Sciences Division, MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune 411004, India.
Bioresour Technol. 2011 Jun;102(11):6625-8. doi: 10.1016/j.biortech.2011.03.054. Epub 2011 Mar 22.
Characterisation of polyhydroxyalkanoate (PHA) film produced by haloalkalitolerant Halomonas campisalis (MCM B-1027) in 14L SS fermenter revealed it to have composition of monomer units, HB:HV as 96:4 as analysed by (1)H NMR indicating the PHA as a co-polymer of PHB-co-PHV, molecular weight by gel permeation chromatography as 2.08 × 10(6), melting temperature 166.51°C, tensile strength 18.8 MPa; two relaxations namely beta transition corresponding to the glass rubber transition and alpha transition corresponding to crystalline relaxation by Dynamic Mechanical Thermal analysis and only one relaxation corresponding to MWS interfacial polarisation with activation energy of 129 kJ/mol by broadband dielectric spectroscopy. Optical microscopic studies showed typical Maltese-cross pattern of spherulites. The PHA film was found to be biodegradable by standard ASTM method as well as by soil burial method. The leak proof polymer bags prepared from the film could be used as a packaging material.
用 14L 发酵罐中的耐盐碱性盐单胞菌（MCM B-1027）生产的聚羟基烷酸酯（PHA）薄膜的特性研究表明，通过（1）H NMR 分析其单体单元组成 HB:HV 为 96:4，表明 PHA 是 PHB-co-PHV 的共聚物，通过凝胶渗透色谱法测得的分子量为 2.08 × 10（6），熔点为 166.51°C，拉伸强度为 18.8 MPa；动态力学热分析表明存在两种弛豫，分别对应于玻璃橡胶转变的β转变和对应于结晶松弛的α转变，而宽带介电光谱法则表明仅存在对应于 MWS 界面极化的一个弛豫，其活化能为 129 kJ/mol。光学显微镜研究显示出典型的马耳他十字形球晶图案。根据标准 ASTM 方法和土壤掩埋法，发现 PHA 薄膜可生物降解。由该薄膜制备的防漏聚合物袋可用作包装材料。
