Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Udupi, Karnataka, 576104, India.
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Udupi, Karnataka, 576104, India.
Arch Microbiol. 2021 Dec;203(10):5993-6005. doi: 10.1007/s00203-021-02554-6. Epub 2021 Sep 22.
Microorganisms have been contemplated as a promising source for the inexhaustible synthesis of many novel materials utilizing renewable sources. Among many of such products, polyhydroxyalkanoate (PHA) remains as an essential biodegradable polymer with functions similar to conventional plastics. Bacillus endophyticus is capable of accumulating biopolymer PHA in nutrient limiting conditions with excess of carbon source. Screening and optimizing the parameters for increased PHA production was done statistically. The optimized medium gave a maximum yield of 46.57% which was in well agreement with the given predicted value provided by response surface methodology model yield of 47.02%. Optimal media conditions when extrapolated in bioreactor gave an even higher production percentage of 49.9. This is the first report highlighting 49% of polyhydroxybutyrate statistically using sucrose as a source. The main highlight of the study was the use of wild type strain for producing high quality PHA using simple carbon source which can be a starting platform for using this strain for large scale PHA production industrially. FTIR and HNMR analysis confirmed the polymer produced.
微生物被认为是利用可再生资源合成许多新型材料的有前途的来源。在众多此类产品中，聚羟基烷酸酯（PHA）仍然是一种必不可少的可生物降解聚合物，其功能与传统塑料相似。内生芽孢杆菌能够在营养限制条件下积累生物聚合物 PHA，同时存在过量的碳源。通过统计学方法对提高 PHA 产量的参数进行了筛选和优化。优化后的培养基的最大产率为 46.57%，与响应面方法模型产率 47.02%提供的给定预测值非常吻合。在生物反应器中推断出的最佳培养基条件甚至给出了 49.9%的更高产量。这是首次使用蔗糖作为来源，通过统计方法报告 49%的聚羟基丁酸酯。该研究的主要亮点是使用野生型菌株使用简单的碳源生产高质量的 PHA，这可以为大规模工业生产 PHA 提供使用该菌株的起点。FTIR 和 HNMR 分析证实了所生产的聚合物。
