Soy protein- and casein-based weaning diets differ in effects on food intake and blood glucose regulation in male Wistar rats.

The effect of weaning male Wistar rats to AIN-93G diets based on casein (C) and soy protein (S) on blood glucose and food intake (FI) regulation was determined. In experiment 1, male Wistar rats (n = 21 per group) received either C or S AIN-93G diets for 7 weeks. In experiment 2, 3 groups of rats were formed (n = 21 per group). The C followed by the S diet group (CS) was weaned to the C diet for 6 weeks followed by the S diet for another 7 weeks. Diet sequence was the reverse for the S followed by the C diet group (SC). The control group (CC) received the C diet throughout 13 weeks. Body weight and cumulative FI were not affected by diet in either experiment. In experiment 1, in fasted rats, S preloads reduced FI for 1 hour more in the C diet group (P < .05), but response to C preloads was not affected by diet. A cholecystokinin A receptor blocker prevented FI reduction by S in rats fed C but not S diet (P < .05). At week 7, rats fed the S diet had higher plasma insulin (67%) (P < .005), glucose (30%) (P < .05) and homeostatic model assessment of insulin resistance index (75%) (P < .005). In experiment 2, FI at weeks 6 and 12 was, again, suppressed most strongly by S preloads in rats fed the C diet (P < .05). At week 13, S and C preloads increased insulin and the insulin/glucose ratio (P < .05), but no differences were found due to preload or diet composition. In conclusion, differences in the effects of first diet exposure to the AIN-93G diets on blood glucose did not persist through either diet change or time. In contrast, protein composition of the most recent diet, but not time, affected FI regulation in response to protein preloads.