Laboratory for Parasitology, Department of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
Vet Parasitol. 2011 Aug 25;180(3-4):287-91. doi: 10.1016/j.vetpar.2011.03.020. Epub 2011 Mar 21.
The distribution of Toxocara cati larvae in the organs of chickens (n=31), experimentally inoculated with 3000 embryonated eggs, was examined 1, 2, 3, 7, 29, 86 and 175-176 days post-infection (dpi), and the infectivity of recovered larvae was evaluated by bioassay in mice. The duodenum, liver, lungs, heart, brain, pectoral muscles (white meat), and hindlimb muscles (red meat) of the chickens were HCl-pepsin digested for larval recovery. Larvae were recovered from all chickens [mean=220.4 ± 114.9 (SD)], and although no decrease of total larval recovery was observed over time, predilection sites changed: liver (92.6% of larval recovery) at 1 dpi; lungs (77.3%) and liver (20.9%) at 2 dpi; lungs (80.8%), muscles (9.4%), and liver (8.5%) at 3 dpi; muscles (52.0%) and lungs (45.6%) at 7 dpi, whereafter most larvae were recovered from muscles: 29 dpi (99.5%), 86 dpi (99.3%) and 175-176 dpi (99.6%). In the bioassay, 52.9% of larvae from 175 to 176 days old infections in chickens established in mice, which demonstrates that T. cati larvae retain infective in the muscles of chickens for half a year. These results highlight the zoonotic potential of poultry meat as a causative agent of human toxocarosis.
猫蛔虫幼虫在实验感染 3000 枚卵的鸡（n=31）器官中的分布，于感染后 1、2、3、7、29、86 和 175-176 天进行检查，并通过生物测定评估回收幼虫的感染力。鸡的十二指肠、肝脏、肺脏、心脏、大脑、胸肌（白肉）和后肢肌肉（红肉）用 HCl-胃蛋白酶消化以回收幼虫。从所有鸡中回收了幼虫[平均值=220.4±114.9（SD）]，尽管总幼虫回收率随时间没有下降，但偏好部位发生了变化：1 天龄时为肝脏（92.6%的幼虫回收率）；2 天龄时为肺脏（77.3%）和肝脏（20.9%）；3 天龄时为肺脏（80.8%）、肌肉（9.4%）和肝脏（8.5%）；7 天龄时为肌肉（52.0%）和肺脏（45.6%），此后大部分幼虫从肌肉中回收：29 天龄（99.5%）、86 天龄（99.3%）和 175-176 天龄（99.6%）。在生物测定中，从感染鸡的 175-176 天龄的幼虫中，有 52.9%在小鼠中建立了感染，这表明猫蛔虫幼虫在鸡的肌肉中保持感染性长达半年。这些结果突出了禽肉作为人类弓蛔虫病病原体的人畜共患病潜力。
