Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, USA.
Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6893-8. doi: 10.1073/pnas.1010894108. Epub 2011 Apr 11.
Quantitative resistance to plant pathogens, controlled by multiple loci of small effect, is important for food production, food security, and food safety but is poorly understood. To gain insights into the genetic architecture of quantitative resistance in maize, we evaluated a 5,000-inbred-line nested association mapping population for resistance to northern leaf blight, a maize disease of global economic importance. Twenty-nine quantitative trait loci were identified, and most had multiple alleles. The large variation in resistance phenotypes could be attributed to the accumulation of numerous loci of small additive effects. Genome-wide nested association mapping, using 1.6 million SNPs, identified multiple candidate genes related to plant defense, including receptor-like kinase genes similar to those involved in basal defense. These results are consistent with the hypothesis that quantitative disease resistance in plants is conditioned by a range of mechanisms and could have considerable mechanistic overlap with basal resistance.
对植物病原体的定量抗性由多个微效基因座控制，对粮食生产、粮食安全和食品安全至关重要，但人们对此知之甚少。为了深入了解玉米中定量抗性的遗传结构，我们评估了一个由 5000 个自交系组成的嵌套关联作图群体对北方叶斑病的抗性，北方叶斑病是一种具有全球经济重要性的玉米病害。鉴定出 29 个数量性状位点，大多数具有多个等位基因。抗性表型的巨大差异可归因于许多具有累加效应的微小基因座的积累。利用 160 万个 SNP 进行全基因组嵌套关联作图，鉴定出与植物防御相关的多个候选基因，包括与基础防御相关的类受体激酶基因。这些结果与植物中定量疾病抗性由一系列机制决定的假设一致，并且可能与基础抗性具有相当大的机制重叠。
