Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
PLoS One. 2019 Jul 30;14(7):e0216438. doi: 10.1371/journal.pone.0216438. eCollection 2019.
Successful antibiotic treatment of infections relies on accurate and rapid identification of the infectious agents. Pseudomonas aeruginosa is implicated in a wide range of human infections that mostly become complicated and life threating, especially in immunocompromised and critically ill patients. Conventional microbiological methods take more than three days to obtain accurate results. Pyocyanin is a distinctive electroactive biomarker for Pseudomonas aeruginosa. Here, we have prepared polyaniline/gold nanoparticles decorated ITO electrode and tested it to establish a rapid, diagnostic and highly sensitive pyocyanin sensor in a culture of Pseudomonas aeruginosa clinical isolates with high selectivity for traces of pyocyanin when measured in the existence of different interferences like vitamin C, uric acid, and glucose. The scanning electron microscopy and cyclic voltammetry techniques were used to characterize the morphology and electrical conductivity of the constructed electrode. The determined linear range for pyocyanin detection was from 238 μM to 1.9 μM with a detection limit of 500 nM. Compared to the screen-printed electrode used before, the constructed electrode showed a 4-fold enhanced performance. Furthermore, PANI/Au NPs/ITO modified electrodes have demonstrated the ability to detect pyocyanin directly in Pseudomonas aeruginosa culture without any potential interference with other species.
抗生素治疗的成功依赖于对感染病原体的准确和快速鉴定。铜绿假单胞菌可导致广泛的人类感染，这些感染大多变得复杂且具有生命威胁，特别是在免疫功能低下和重症患者中。传统的微生物学方法需要超过三天才能获得准确的结果。绿脓菌素是铜绿假单胞菌的一种独特的电活性生物标志物。在这里，我们制备了聚邻苯二胺/金纳米粒子修饰的 ITO 电极，并对其进行了测试，以建立一种快速、诊断和高灵敏度的绿脓菌素传感器，该传感器对铜绿假单胞菌临床分离株的培养物中的痕量绿脓菌素具有高度选择性，在存在不同干扰物（如维生素 C、尿酸和葡萄糖）时，测量的绿脓菌素的选择性也很高。扫描电子显微镜和循环伏安技术用于表征构建电极的形态和电导率。绿脓菌素检测的测定线性范围为 238 μM 至 1.9 μM，检测限为 500 nM。与以前使用的丝网印刷电极相比，构建的电极显示出 4 倍的性能增强。此外，PANI/Au NPs/ITO 修饰电极已证明能够直接在铜绿假单胞菌培养物中检测绿脓菌素，而不会对其他物种产生任何潜在干扰。
