Rodriguez-Cordoba S, Regueiro J R, Arnaiz-Villena A
Department of Immunology, C.S.I.C., Madrid, Spain.
J Reprod Immunol. 1990 Oct;18(3):237-45. doi: 10.1016/0165-0378(90)90046-9.
Purified human spermatozoa do not regularly stimulate lymphocytes in spermlymphocyte cultures (SLC). This lack of consistent stimulation was found not to be dependent on sperm/lymphocyte ratio or on culture peak response time. A few weak stimulations obtained are not HLA-D or sex dependent. Contaminating seminal cells other than spermatozoa (SC non-SZ) may be responsible for the high stimulations found in SLC by others, since, in our hands, purified suspensions of SC non-SZ stimulate allogeneic lymphocytes in an HLA-D-dependent fashion, and such responses are abrogated by anti-HLA-DR monoclonal antibodies. These functional data confirm our previous finding of HLA-DR molecules on SC non-SZ by absorptions (and a lack of expression on spermatozoa) and suggest the concomitant expression of HLA-DQ and -DP products.
纯化的人类精子在精子 - 淋巴细胞培养物（SLC）中通常不会刺激淋巴细胞。发现这种缺乏一致刺激的情况不依赖于精子/淋巴细胞比例或培养峰值反应时间。获得的一些微弱刺激不依赖于HLA - D或性别。除精子外的污染性精细胞（非精子的精细胞，SC non - SZ）可能是其他人在SLC中发现的高刺激的原因，因为在我们的实验中，纯化的SC non - SZ悬浮液以依赖HLA - D的方式刺激同种异体淋巴细胞，并且此类反应可被抗HLA - DR单克隆抗体消除。这些功能数据证实了我们之前通过吸收法在SC non - SZ上发现HLA - DR分子（而精子上缺乏表达）的结果，并提示HLA - DQ和 - DP产物的伴随表达。
