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拭子材料和运输培养基对伪狂犬病病毒的影响。

Effects of swab materials and transport media on Aujeszky's disease virus.

作者信息

Lloyd G, Baskerville A

出版信息

Res Vet Sci. 1978 Sep;25(2):257-9.

PMID:214836
Abstract

The effect of swab materials on the recovery of Aujeszky's disease virus from various transport media held at 4 degrees C was investigated over a five day period. No significant loss in infectivity was found in control preparations, approximately 50 per cent of infectivity was recovered from fluids containing applicator wire and approximately 10 per cent from fluids containing polyester fibre or cotton wool. Virus recovery from fluids containing wooden applicator sticks ranged from no virus recovery in protein free media to from 1 to 10 per cent in protein containing media. Freezing followed by thawing was the most effective of the physical methods used for eluting virus from swab materials.

摘要

在五天时间内,研究了拭子材料对在4摄氏度保存的各种运输培养基中奥耶斯基氏病病毒回收率的影响。对照制剂未发现感染力有显著损失,含涂抹器金属丝的液体中约50%的感染力得以恢复,含聚酯纤维或脱脂棉的液体中约10%的感染力得以恢复。从含木制涂抹棒的液体中回收病毒的情况,在无蛋白培养基中未回收病毒,在含蛋白培养基中为1%至10%。冷冻后解冻是从拭子材料中洗脱病毒最有效的物理方法。

相似文献

1
Effects of swab materials and transport media on Aujeszky's disease virus.拭子材料和运输培养基对伪狂犬病病毒的影响。
Res Vet Sci. 1978 Sep;25(2):257-9.
2
Effects of swab materials on infectious bovine rhinotracheitis virus.
Am J Vet Res. 1976 Jun;37(6):707-8.
3
Influence of environmental factors upon the survival of Aujeszky's disease virus.
Res Vet Sci. 1981 Jul;31(1):32-6.
4
Rapid diagnosis of Aujeszky's disease in pigs by immunofluorescence.
Res Vet Sci. 1984 Mar;36(2):235-9.
5
A novel concept for the control of Aujeszky's disease: experiences in two vaccinated pig herds.一种控制奥耶斯基氏病的新观念:两个接种疫苗猪群的经验
Vet Rec. 1990 Feb 17;126(7):159-63.
6
[Development of a production method of reproducing the Aujeszky's disease virus].[一种繁殖奥耶斯基氏病病毒生产方法的研发]
Veterinariia. 1975 Oct(10):39-42.
7
[Use of the plaque method for isolating Aujeszky's disease virus from meat, organs and meat products].[使用噬斑法从肉类、器官和肉制品中分离奥耶斯基氏病病毒]
Vet Med Nauki. 1977;14(6):79-83.
8
The use of the indirect immunoperoxidase method for the detection of Aujeszky's disease virus in cell culture.间接免疫过氧化物酶法在细胞培养中检测奥耶斯基病病毒的应用。
Zentralbl Veterinarmed B. 1979 Apr;26(3):253-6. doi: 10.1111/j.1439-0450.1979.tb00813.x.
9
Safety of an Aujeszky's disease vaccine based on deletion mutant strain 783 which does not express thymidine kinase and glycoprotein I.基于不表达胸苷激酶和糖蛋白I的缺失突变株783的奥耶斯基氏病疫苗的安全性。
Vet Rec. 1990 Nov 3;127(18):443-6.
10
[Aujeszky's disease virus accumulation in a culture of isolated porcine kidneys].[奥耶斯基氏病病毒在离体猪肾培养物中的积聚]
Veterinariia. 1975 Apr(4):41-3.

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