Program in Developmental Biology, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America.
PLoS One. 2011 Apr 5;6(4):e18616. doi: 10.1371/journal.pone.0018616.
Generally, epithelial cells must organize in three dimensions to form functional tissue sheets. Here we investigate one such sheet, the Drosophila embryonic epidermis, and the morphogenetic processes organizing cells within it. We report that epidermal morphogenesis requires the proper distribution of the apical polarity determinant aPKC. Specifically, we find roles for the kinases GSK3 and aPKC in cellular alignment, asymmetric protein distribution, and adhesion during the development of this polarized tissue. Finally, we propose a model explaining how regulation of aPKC protein levels can reorganize both adhesion and the cytoskeleton.
通常，上皮细胞必须在三维空间中组织以形成功能性组织片。在这里，我们研究了这样一个组织片，即果蝇胚胎表皮，以及组织内细胞的形态发生过程。我们报告说，表皮形态发生需要顶端极性决定因素 aPKC 的正确分布。具体而言，我们发现激酶 GSK3 和 aPKC 在这种极化组织的细胞排列、不对称蛋白分布和粘附过程中发挥作用。最后，我们提出了一个模型，解释了 aPKC 蛋白水平的调节如何重新组织粘附和细胞骨架。
