• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

拍摄快速 HIV 检测结果试点新型质量评估和培训计划。

Photographed rapid HIV test results pilot novel quality assessment and training schemes.

机构信息

NRL, St Vincent's Institute, Melbourne, Victoria, Australia.

出版信息

PLoS One. 2011 Mar 31;6(3):e18294. doi: 10.1371/journal.pone.0018294.

DOI:10.1371/journal.pone.0018294
PMID:21483842
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3069085/
Abstract

HIV rapid diagnostic tests (RDTs) are now used widely in non-laboratory settings by non-laboratory-trained operators. Quality assurance programmes are essential in ensuring the quality of HIV RDT outcomes. However, there is no cost-effective means of supplying the many operators of RDTs with suitable quality assurance schemes. Therefore, it was examined whether photograph-based RDT results could be used and correctly interpreted in the non-laboratory setting. Further it was investigated if a single training session improved the interpretation skills of RDT operators. The photographs were interpreted, a 10-minute tutorial given and then a second interpretation session was held. It was established that the results could be read with accuracy. The participants (n=75) with a range of skills interpreted results (>80% concordance with reference results) from a panel of 10 samples (three negative and seven positive) using four RDTs. Differences in accuracy of interpretation before and after the tutorial were marked in some cases. Training was more effective for improving the accurate interpretation of more complex results, e.g. results with faint test lines or for multiple test lines, and especially for improving interpretation skills of inexperienced participants. It was demonstrated that interpretation of RDTs was improved using photographed results allied to a 10-minute training session. It is anticipated that this method could be used for training but also for quality assessment of RDT operators without access to conventional quality assurance or training schemes requiring wet samples.

摘要

艾滋病毒快速诊断检测(RDT)现在由非实验室训练的操作人员在非实验室环境中广泛使用。质量保证计划对于确保 HIV RDT 结果的质量至关重要。然而,没有一种具有成本效益的方法可以为众多 RDT 操作人员提供合适的质量保证计划。因此,研究了是否可以在非实验室环境中使用基于照片的 RDT 结果并正确解释这些结果。进一步研究了单次培训是否可以提高 RDT 操作人员的解释技能。对照片进行了解释,进行了 10 分钟的教程,然后进行了第二次解释。结果表明,可以准确读取结果。有一定技能的参与者(n=75)使用四种 RDT 从 10 个样本(三个阴性和七个阳性)面板中解释结果(与参考结果的一致性>80%)。在某些情况下,教程前后解释准确性的差异非常明显。培训对于提高更复杂结果(例如测试线较浅或有多个测试线的结果)的准确解释更为有效,尤其是对于提高经验不足的参与者的解释技能。结果表明,通过拍摄 RDT 照片并结合 10 分钟的培训课程,可以提高 RDT 的解释能力。预计这种方法可以用于培训,也可以用于对无法获得传统质量保证或需要湿样本的培训计划的 RDT 操作人员进行质量评估。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/42adb1891a58/pone.0018294.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/f71f48500540/pone.0018294.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/872fa8e436d5/pone.0018294.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/a4b9e4a1d198/pone.0018294.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/42adb1891a58/pone.0018294.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/f71f48500540/pone.0018294.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/872fa8e436d5/pone.0018294.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/a4b9e4a1d198/pone.0018294.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/42adb1891a58/pone.0018294.g004.jpg

相似文献

1
Photographed rapid HIV test results pilot novel quality assessment and training schemes.拍摄快速 HIV 检测结果试点新型质量评估和培训计划。
PLoS One. 2011 Mar 31;6(3):e18294. doi: 10.1371/journal.pone.0018294.
2
Photo-based External Quality Assessment of Malaria rapid diagnostic tests in a non-endemic setting.基于照片的疟疾快速诊断检测在非流行地区的外部质量评估。
PLoS One. 2018 Aug 31;13(8):e0201622. doi: 10.1371/journal.pone.0201622. eCollection 2018.
3
Assessing proficiency of interpretation of rapid human immunodeficiency virus assays in nonlaboratory settings: ensuring quality of testing.评估非实验室环境下快速人类免疫缺陷病毒检测结果判读的熟练度:确保检测质量。
J Clin Microbiol. 2008 May;46(5):1692-7. doi: 10.1128/JCM.01761-07. Epub 2008 Mar 19.
4
SMS photograph-based external quality assessment of reading and interpretation of malaria rapid diagnostic tests in the Democratic Republic of the Congo.刚果民主共和国基于短信照片的疟疾快速诊断检测阅读与判读外部质量评估。
Malar J. 2015 Jan 28;14:26. doi: 10.1186/s12936-014-0535-9.
5
External quality assessment on the use of malaria rapid diagnostic tests in a non-endemic setting.在非疟疾流行地区使用疟疾快速诊断检测的外部质量评估。
Malar J. 2010 Dec 13;9:359. doi: 10.1186/1475-2875-9-359.
6
Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities.坦桑尼亚农村军事医疗机构中 Deki Reader 疟疾快速诊断检测的视觉解读与自动解读比较。
Malar J. 2018 May 29;17(1):214. doi: 10.1186/s12936-018-2363-9.
7
Development of an approach to monitor the manufacturing consistency of HIV rapid diagnostic tests: Panel qualification and potential impact on country programs.开发一种监测 HIV 快速诊断检测制造一致性的方法:小组资格认证及对国家项目的潜在影响。
PLoS One. 2023 Apr 10;18(4):e0284175. doi: 10.1371/journal.pone.0284175. eCollection 2023.
8
The use of Fionet technology for external quality control of malaria rapid diagnostic tests and monitoring health workers' performance in rural military health facilities in Tanzania.利用 Fionet 技术对疟疾快速诊断检测进行外部质量控制,并监测坦桑尼亚农村军事卫生机构中卫生工作者的表现。
PLoS One. 2018 Dec 27;13(12):e0208583. doi: 10.1371/journal.pone.0208583. eCollection 2018.
9
Dilution testing using rapid diagnostic tests in a HIV diagnostic algorithm: a novel alternative for confirmation testing in resource limited settings.在HIV诊断算法中使用快速诊断测试进行稀释检测:一种在资源有限环境中进行确认检测的新方法。
Virol J. 2015 May 14;12:75. doi: 10.1186/s12985-015-0306-4.
10
Field assessment of dried Plasmodium falciparum samples for malaria rapid diagnostic test quality control and proficiency testing in Ethiopia.埃塞俄比亚用于疟疾快速诊断检测质量控制和能力验证的恶性疟原虫干血样现场评估
Malar J. 2015 Jan 21;14:11. doi: 10.1186/s12936-014-0524-z.

引用本文的文献

1
Development of an approach to monitor the manufacturing consistency of HIV rapid diagnostic tests: Panel qualification and potential impact on country programs.开发一种监测 HIV 快速诊断检测制造一致性的方法:小组资格认证及对国家项目的潜在影响。
PLoS One. 2023 Apr 10;18(4):e0284175. doi: 10.1371/journal.pone.0284175. eCollection 2023.
2
Improving the quality of HIV rapid testing in Ghana using the dried tube specimen-based proficiency testing program.利用干血斑标本能力验证项目提高加纳 HIV 快速检测质量。
PLoS One. 2022 Oct 14;17(10):e0264105. doi: 10.1371/journal.pone.0264105. eCollection 2022.
3
Evaluation of the Performance of Health Extension Workers in HIV-1/2 Screening Tests: A Comparative Cross-Sectional Study, Addis Ababa, Ethiopia.

本文引用的文献

1
Inaccurate diagnosis of HIV-1 group M and O is a key challenge for ongoing universal access to antiretroviral treatment and HIV prevention in Cameroon.在喀麦隆,艾滋病毒 1 型 M 和 O 群的不准确诊断是持续普及抗逆转录病毒治疗和艾滋病毒预防的一个关键挑战。
PLoS One. 2009 Nov 6;4(11):e7702. doi: 10.1371/journal.pone.0007702.
2
Immunoassays for the diagnosis of HIV: meeting future needs by enhancing the quality of testing.用于 HIV 诊断的免疫测定:通过提高检测质量来满足未来的需求。
Future Microbiol. 2009 Oct;4(8):963-82. doi: 10.2217/fmb.09.77.
3
Suitability of simple human immunodeficiency virus rapid tests in clinical trials in community-based clinic settings.
埃塞俄比亚亚的斯亚贝巴卫生推广工作者在HIV-1/2筛查试验中的表现评估:一项比较性横断面研究
AIDS Res Treat. 2020 Nov 19;2020:7841352. doi: 10.1155/2020/7841352. eCollection 2020.
4
External quality assessment for HIV rapid tests: challenges and opportunities in Haiti.艾滋病病毒快速检测的外部质量评估:海地面临的挑战与机遇
BMJ Glob Health. 2018 Nov 21;3(6):e001074. doi: 10.1136/bmjgh-2018-001074. eCollection 2018.
5
A rapid ultrasound particle agglutination method for HIV antibody detection: Comparison with conventional rapid HIV tests.一种用于 HIV 抗体检测的快速超声粒子凝集法:与传统快速 HIV 检测的比较。
J Virol Methods. 2017 Nov;249:38-47. doi: 10.1016/j.jviromet.2017.08.015. Epub 2017 Aug 24.
6
Accuracy in HIV Rapid Testing among Laboratory and Non-laboratory Personnel in Zambia: Observations from the National HIV Proficiency Testing System.赞比亚实验室及非实验室工作人员中HIV快速检测的准确性:来自国家HIV能力验证检测系统的观察结果
PLoS One. 2016 Jan 8;11(1):e0146700. doi: 10.1371/journal.pone.0146700. eCollection 2016.
7
Task-Shifting and Quality of HIV Testing Services: Experiences from a National Reference Hospital in Zambia.任务转移与艾滋病毒检测服务质量:赞比亚一家国家级参考医院的经验
PLoS One. 2015 Nov 25;10(11):e0143075. doi: 10.1371/journal.pone.0143075. eCollection 2015.
8
HIV and syphilis testing preferences among men who have sex with men in South China: a qualitative analysis to inform sexual health services.中国南方男男性行为者对艾滋病毒和梅毒检测的偏好:一项为性健康服务提供信息的定性分析
PLoS One. 2015 Apr 13;10(4):e0124161. doi: 10.1371/journal.pone.0124161. eCollection 2015.
9
Leukodepletion as a point-of-care method for monitoring HIV-1 viral load in whole blood.白细胞去除作为一种即时检测方法用于监测全血中的HIV-1病毒载量。
J Clin Microbiol. 2015 Apr;53(4):1080-6. doi: 10.1128/JCM.02853-14. Epub 2014 Nov 26.
10
Field evaluation of an automated RDT reader and data management device for Plasmodium falciparum/Plasmodium vivax malaria in endemic areas of Colombia.哥伦比亚流行地区用于疟原虫/间日疟原虫疟疾的自动 RDT 读取器和数据管理设备的现场评估。
Malar J. 2014 Mar 10;13:87. doi: 10.1186/1475-2875-13-87.
简易人类免疫缺陷病毒快速检测在社区诊所环境下临床试验中的适用性。
J Clin Microbiol. 2009 Apr;47(4):1058-62. doi: 10.1128/JCM.01998-08. Epub 2009 Feb 25.
4
The evaluation of a rapid in situ HIV confirmation test in a programme with a high failure rate of the WHO HIV two-test diagnostic algorithm.在世界卫生组织艾滋病毒两步检测诊断算法失败率较高的项目中对一种快速原位艾滋病毒确认检测进行评估。
PLoS One. 2009;4(2):e4351. doi: 10.1371/journal.pone.0004351. Epub 2009 Feb 6.
5
Implementing rapid HIV testing in outreach and community settings: results from an advancing HIV prevention demonstration project conducted in seven U.S. cities.在美国七个城市开展的一项推进艾滋病预防示范项目中,在推广和社区环境中实施快速艾滋病毒检测:结果报告
Public Health Rep. 2008 Nov-Dec;123 Suppl 3(Suppl 3):78-85. doi: 10.1177/00333549081230S310.
6
Validation, performance under field conditions, and cost-effectiveness of Capillus HIV-1/HIV-2 and determine HIV-1/2 rapid human immunodeficiency virus antibody assays using sequential and parallel testing algorithms in Tanzania.在坦桑尼亚使用序贯和平行检测算法对Capillus HIV-1/HIV-2及确定HIV-1/2快速人类免疫缺陷病毒抗体检测进行验证、现场条件下的性能评估及成本效益分析。
J Clin Microbiol. 2008 Dec;46(12):3946-51. doi: 10.1128/JCM.01045-08. Epub 2008 Oct 15.
7
Rapid vs. standard HIV testing in bathhouses: what is gained and lost?浴室中快速与标准 HIV 检测:得失如何?
AIDS Behav. 2010 Jun;14(3):688-96. doi: 10.1007/s10461-008-9442-9. Epub 2008 Aug 26.
8
Impact of round-the-clock, rapid oral fluid HIV testing of women in labor in rural India.印度农村地区对分娩期妇女进行全天候快速口服液体HIV检测的影响
PLoS Med. 2008 May 6;5(5):e92. doi: 10.1371/journal.pmed.0050092.
9
Assessing proficiency of interpretation of rapid human immunodeficiency virus assays in nonlaboratory settings: ensuring quality of testing.评估非实验室环境下快速人类免疫缺陷病毒检测结果判读的熟练度:确保检测质量。
J Clin Microbiol. 2008 May;46(5):1692-7. doi: 10.1128/JCM.01761-07. Epub 2008 Mar 19.
10
Evaluation and implementation of rapid HIV tests: the experience in 11 African countries.快速艾滋病毒检测的评估与实施:11个非洲国家的经验
AIDS Res Hum Retroviruses. 2007 Dec;23(12):1491-8. doi: 10.1089/aid.2007.0020.