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拍摄快速 HIV 检测结果试点新型质量评估和培训计划。

Photographed rapid HIV test results pilot novel quality assessment and training schemes.

机构信息

NRL, St Vincent's Institute, Melbourne, Victoria, Australia.

出版信息

PLoS One. 2011 Mar 31;6(3):e18294. doi: 10.1371/journal.pone.0018294.

Abstract

HIV rapid diagnostic tests (RDTs) are now used widely in non-laboratory settings by non-laboratory-trained operators. Quality assurance programmes are essential in ensuring the quality of HIV RDT outcomes. However, there is no cost-effective means of supplying the many operators of RDTs with suitable quality assurance schemes. Therefore, it was examined whether photograph-based RDT results could be used and correctly interpreted in the non-laboratory setting. Further it was investigated if a single training session improved the interpretation skills of RDT operators. The photographs were interpreted, a 10-minute tutorial given and then a second interpretation session was held. It was established that the results could be read with accuracy. The participants (n=75) with a range of skills interpreted results (>80% concordance with reference results) from a panel of 10 samples (three negative and seven positive) using four RDTs. Differences in accuracy of interpretation before and after the tutorial were marked in some cases. Training was more effective for improving the accurate interpretation of more complex results, e.g. results with faint test lines or for multiple test lines, and especially for improving interpretation skills of inexperienced participants. It was demonstrated that interpretation of RDTs was improved using photographed results allied to a 10-minute training session. It is anticipated that this method could be used for training but also for quality assessment of RDT operators without access to conventional quality assurance or training schemes requiring wet samples.

摘要

艾滋病毒快速诊断检测(RDT)现在由非实验室训练的操作人员在非实验室环境中广泛使用。质量保证计划对于确保 HIV RDT 结果的质量至关重要。然而,没有一种具有成本效益的方法可以为众多 RDT 操作人员提供合适的质量保证计划。因此,研究了是否可以在非实验室环境中使用基于照片的 RDT 结果并正确解释这些结果。进一步研究了单次培训是否可以提高 RDT 操作人员的解释技能。对照片进行了解释,进行了 10 分钟的教程,然后进行了第二次解释。结果表明,可以准确读取结果。有一定技能的参与者(n=75)使用四种 RDT 从 10 个样本(三个阴性和七个阳性)面板中解释结果(与参考结果的一致性>80%)。在某些情况下,教程前后解释准确性的差异非常明显。培训对于提高更复杂结果(例如测试线较浅或有多个测试线的结果)的准确解释更为有效,尤其是对于提高经验不足的参与者的解释技能。结果表明,通过拍摄 RDT 照片并结合 10 分钟的培训课程,可以提高 RDT 的解释能力。预计这种方法可以用于培训,也可以用于对无法获得传统质量保证或需要湿样本的培训计划的 RDT 操作人员进行质量评估。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa0f/3069085/f71f48500540/pone.0018294.g001.jpg

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