Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, Seoul, 120-749, Korea.
Genes Nutr. 2011 May;6(2):117-23. doi: 10.1007/s12263-010-0195-5. Epub 2010 Dec 5.
Reactive oxygen species (ROS) are known to be involved in the pathogenesis of acute and chronic pancreatitis. The cholecystokinin (CCK) analog cerulein causes pathophysiological, morphological, and biochemical events similar to those observed in human acute pancreatitis. The oxidant-sensitive transcription factor NF-κB plays a critical role in the development of cerulein pancreatitis by regulating the expression of pro-inflammatory cytokines in the pancreas. Lycopene has an anti-oxidant effect in various cells. In the present study, we investigated whether cerulein induces NF-κB activation and IL-6 expression in pancreatic acinar cells and whether lycopene inhibits these events. NF-κB-DNA-binding activity was determined by electrophoretic mobility shift assay, and mRNA expression was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR analyses. The IL-6 concentration in the medium was determined by enzyme-linked immunosorbent assay. Our results showed that cerulein induced IL-6 expression in a time-dependent manner. NF-κB-DNA-binding activity and intracellular levels of ROS in pancreatic acinar cells were increased by cerulein. Lycopene inhibited the cerulein-induced increase in intracellular ROS, NF-κB activation, and IL-6 expression in pancreatic acinar cells in a dose-dependent manner. In conclusion, lycopene may be beneficial in the prevention and/or treatment of acute pancreatitis by inhibiting the activation of NF-κB and the expression of inflammatory cytokines through reduction in intracellular levels of ROS in pancreatic acinar cells.
活性氧（ROS）已知参与急性和慢性胰腺炎的发病机制。胆囊收缩素（CCK）类似物蛙皮素引起类似于人类急性胰腺炎的病理生理、形态和生化事件。氧化敏感转录因子 NF-κB 通过调节胰腺中促炎细胞因子的表达，在蛙皮素胰腺炎的发生发展中起关键作用。番茄红素在各种细胞中具有抗氧化作用。在本研究中，我们研究了蛙皮素是否诱导胰腺腺泡细胞中 NF-κB 的激活和 IL-6 的表达，以及番茄红素是否抑制这些事件。通过电泳迁移率变动分析测定 NF-κB-DNA 结合活性，通过逆转录-聚合酶链反应（RT-PCR）和实时 RT-PCR 分析分析 mRNA 表达。通过酶联免疫吸附试验测定培养基中 IL-6 的浓度。我们的结果表明，蛙皮素以时间依赖性方式诱导 IL-6 的表达。NF-κB-DNA 结合活性和胰腺腺泡细胞内 ROS 的水平增加了蛙皮素。番茄红素以剂量依赖性方式抑制胰腺腺泡细胞中 ROS 、 NF-κB 激活和 IL-6 表达的增加。总之，番茄红素可能通过降低胰腺腺泡细胞内 ROS 的水平，抑制 NF-κB 的激活和炎症细胞因子的表达，对急性胰腺炎的预防和/或治疗有益。
