Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 22600, People's Republic of China.
J Mol Neurosci. 2011 Oct;45(2):277-83. doi: 10.1007/s12031-011-9518-2. Epub 2011 Apr 12.
Schwann cell precursors differentiating into a myelinating phenotype are critical for peripheral nerve development and regeneration. However, little is known about the underlying molecular mechanisms of Schwann cell differentiation. In the present study, we performed a cyclic adenosine monophosphate-induced Schwann cell differentiation model in vitro. Western blot analysis showed that p27(Kip1) expression was upregulated during the differentiation of Schwann cell, while the inhibition of p27(Kip1) expression by short hairpin RNA-mediated knockdown significantly abolished the expression of promyelinating markers and the alteration of cellular morphology. In addition, immunofluorescence revealed a decrease of p27(Kip1) nuclear staining and a concomitant increase of cytoplasmic staining in differentiated Schwann cells. In summary, our data indicated that p27(Kip1) was a positive regulator of Schwann cell differentiation in vitro.
施万细胞前体细胞分化为髓鞘形成表型对于周围神经的发育和再生至关重要。然而，施万细胞分化的潜在分子机制知之甚少。本研究在体外建立环磷酸腺苷诱导的施万细胞分化模型。Western blot 分析显示，p27(Kip1)表达在施万细胞分化过程中上调，而短发夹 RNA 介导的敲低抑制 p27(Kip1)表达显著消除了前髓鞘形成标记物的表达和细胞形态的改变。此外，免疫荧光显示分化的施万细胞中 p27(Kip1)核染色减少，细胞质染色增加。总之，我们的数据表明 p27(Kip1)是体外施万细胞分化的正向调节因子。
