Division of Craniofacial and Molecular Genetics, Department of Oral and Maxillofacial Pathology, Tufts University School of Dental Medicine, Boston, Massachusetts, USA.
J Biomed Mater Res A. 2011 Jun 15;97(4):414-22. doi: 10.1002/jbm.a.33062. Epub 2011 Apr 11.
Silk scaffolds have been successfully used for a variety of tissue engineering applications due to their biocompatibility, diverse physical characteristics, and ability to support cell attachment and proliferation. Our prior characterization of 4-day postnatal rat tooth bud cells grown on hexafluoro-2-propanol (HFIP) silk scaffolds showed that the silk scaffolds not only supported osteodentin formation, but also guided the size and shape of the formed osteodentin. In this study, interactions between human dental pulp cells and HFIP and aqueous based silk scaffolds were studied under both in vitro and in vivo conditions. Silk scaffold porosity and incorporation of RGD and DMP peptides were examined. We found that the degradation of aqueous based silk is much faster than HFIP based silk scaffolds. Also, HFIP based silk scaffolds supported the soft dental pulp formation better than the aqueous based silk scaffolds. No distinct hard tissue regeneration was found in any of the implants, with or without additional cells. We conclude that alternative silk scaffold materials, and hDSC pre-seeding cell treatments or sorting and enrichment methods, need to be considered for successful dental hard tissue regeneration.
丝素支架由于其生物相容性、多样的物理特性以及支持细胞附着和增殖的能力，已成功应用于各种组织工程应用。我们之前对在六氟异丙醇（HFIP）丝素支架上培养的 4 天龄新生大鼠牙胚细胞的特性进行了表征，结果表明丝素支架不仅支持骨牙本质的形成，而且还指导了形成的骨牙本质的大小和形状。在这项研究中，研究了人牙髓细胞与 HFIP 和水基丝素支架在体外和体内条件下的相互作用。研究了丝素支架的孔隙率以及 RGD 和 DMP 肽的掺入情况。我们发现水基丝素的降解速度明显快于 HFIP 基丝素支架。此外，HFIP 基丝素支架比水基丝素支架更有利于软牙髓的形成。在任何植入物中，无论是否添加额外的细胞，都没有发现明显的硬组织再生。我们得出结论，需要考虑替代的丝素支架材料以及 hDSC 预接种细胞处理或分选和富集方法，以实现成功的牙科硬组织再生。
