Nazhvani Fatemeh Dehghani, Kazempour Setareh, Hosseini Seyed-Mojtaba, Nazhvani Ali Dehghani, Haddadi Pardis
Bone and Joint Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Dent Res J (Isfahan). 2021 Oct 21;18:86. eCollection 2021.
Although missing tooth is not life-threatening, it affects the quality of daily life. Stem cells have emerged as an important player in the generation and maintenance of many tissues. The role of scaffolds has changed from a passive carrier to a bioactive matrix, which can be used to induce cellular behavior. The aim of this study was to determine the possibility of regeneration of dentin-pulp complex with dental pulp stem cells (DPSCs) in an animal model.
In this animal study after extraction of DPSCs and cultivation, 10 types of scaffolds were made by using platelet-rich plasma (PRP), cancellous bone, and collagen pad. They were inserted in different parts of the dog's mouth. After the 4 month, the area was operated, and the scaffolds were removed.
Microscopic examination revealed no sign of cell differentiation and formation of new structures in those models which used collagen scaffolds. However, the dentin-pulp complex emerged in models that the combination of bone scaffolds and PRP or stem cells was used.
Using bone scaffolds in combination with PRP or DPSCs to regenerate dentin-pulp complex in dog helped odontoblastic and pulpal differentiation as well as the formation of predentin and tubular dentin.
虽然缺牙不会危及生命，但它会影响日常生活质量。干细胞已成为许多组织生成和维持过程中的重要参与者。支架的作用已从被动载体转变为生物活性基质，可用于诱导细胞行为。本研究的目的是在动物模型中确定牙髓干细胞（DPSCs）再生牙髓-牙本质复合体的可能性。
在这项动物研究中，提取DPSCs并培养后，使用富血小板血浆（PRP）、松质骨和胶原垫制作了10种类型的支架。将它们植入狗口腔的不同部位。4个月后，对该区域进行手术，并取出支架。
显微镜检查显示，在使用胶原支架的模型中没有细胞分化和新结构形成的迹象。然而，在使用骨支架与PRP或干细胞组合的模型中出现了牙髓-牙本质复合体。
在狗身上使用骨支架与PRP或DPSCs联合再生牙髓-牙本质复合体有助于成牙本质细胞和牙髓分化以及前期牙本质和管状牙本质的形成。
