Department of Dental Anesthesiology and Dental Research Institute, Seoul National University, Seoul, Republic of Korea .
Tissue Eng Part A. 2010 Apr;16(4):1271-9. doi: 10.1089/ten.TEA.2009.0328.
We developed three-dimensional electrospun silk fibroin (ESF) scaffolds with controllable pore size. The purpose of this study was to evaluate ESF scaffolds with pores (P-ESF) for bone regeneration via in vitro and in vivo studies, with a comparison to a commercially available porous three-dimensional polylactic acid (PLA) scaffold. P-ESF supported significantly higher proliferation and alkaline phosphatase activity of osteoblasts than PLA in vitro (p < 0.05). Moreover, higher expression levels of activated adhesion-related proteins, including focal adhesion kinase, were observed in the P-ESF than in PLA, as confirmed by western blot analyses. Microcomputed tomography revealed that 78.30% of the original bone volume was attained in the P-ESF implantation group at 7 weeks after critical bone defect formation in rat calvaria. Comparatively, the PLA implantation group showed only 49.31%. Histological evaluation also showed new bone tissue formation upon P-ESF implantation. Taken together, the P-ESF scaffold may be a good bone substitute for bone regeneration.
我们开发了具有可控孔径的三维丝素蛋白（ESF）支架。本研究旨在通过体外和体内研究评估具有孔的 ESF 支架（P-ESF）在骨再生方面的应用，并与市售的多孔三维聚乳酸（PLA）支架进行比较。体外实验结果表明，P-ESF 支持成骨细胞的增殖和碱性磷酸酶活性明显高于 PLA（p<0.05）。此外，通过 Western blot 分析证实，P-ESF 中黏附相关蛋白的激活表达水平，包括粘着斑激酶，高于 PLA。微计算机断层扫描显示，在大鼠颅骨临界骨缺损形成 7 周后，P-ESF 植入组的原始骨体积恢复率达到 78.30%。相比之下，PLA 植入组仅为 49.31%。组织学评价也表明 P-ESF 植入后有新骨组织形成。综上所述，P-ESF 支架可能是一种良好的骨替代物，可用于骨再生。
