Xu Wan-Peng, Zhang Weibo, Asrican Rose, Kim Hyeon-Joo, Kaplan David L, Yelick Pamela C
Department of Oral and Maxillofacial Pathology, Division of Craniofacial and Molecular Genetics, Tufts University, Boston, Massachusetts, USA.
Tissue Eng Part A. 2008 Apr;14(4):549-57. doi: 10.1089/tea.2007.0227.
Based on the successful use of silk scaffolds in bone tissue engineering, we examined their utility for mineralized dental tissue engineering. Four types of hexafluoroisopropanol (HFIP) silk scaffolds-(250 and 550 microm diameter pores, with or without arginine-glycine-aspartic acid (RGD) peptide) were seeded with cultured 4-day postnatal rat tooth bud cells and grown in the rat omentum for 20 weeks. Analyses of harvested implants revealed the formation of bioengineered mineralized tissue that was most robust in 550 microm pore RGD-containing scaffolds and least robust in 250 microm pore sized scaffolds without RGD. The size and shape of the silk scaffold pores appeared to guide mineralized tissue formation, as revealed using polarized light imaging of collagen fiber alignment along the scaffold surfaces. This study is the first to characterize bioengineered tissues generated from tooth bud cells seeded onto silk scaffolds and indicates that silk scaffolds may be useful in forming mineralized osteodentin of specified sizes and shapes.
基于丝支架在骨组织工程中的成功应用，我们研究了其在矿化牙组织工程中的效用。将四种类型的六氟异丙醇（HFIP）丝支架（孔径为250和550微米，含或不含精氨酸 - 甘氨酸 - 天冬氨酸（RGD）肽）接种培养4天的新生大鼠牙胚细胞，并在大鼠大网膜中培养20周。对收获的植入物进行分析发现，生物工程矿化组织形成，在含RGD的550微米孔径支架中最为显著，而在不含RGD的250微米孔径支架中最不显著。如使用沿支架表面胶原纤维排列的偏振光成像所示，丝支架孔的大小和形状似乎引导矿化组织形成。本研究首次对接种到丝支架上的牙胚细胞产生的生物工程组织进行了表征，并表明丝支架可能有助于形成特定大小和形状的矿化骨样牙本质。
