[人参皂苷Rb1对氧化型低密度脂蛋白诱导的人单核细胞衍生树突状细胞免疫成熟的影响]

[Effect of ginsenoside Rb1 on immune maturation of human monocyte-derived dendritic cells induced by oxidized low-density lipoprotein].

作者信息

Liu Hong-ying, Shi Da-zhuo, Ge Jun-bo

机构信息

Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Mar;31(3):350-4.

PMID:21485077

Abstract

OBJECTIVE

To examine the effect of ginsenoside Rb1 (GRb1) on the immune maturation of monocyte-derived dendritic cells (DCs) induced by oxidized low-density lipoprotein (OX-LDL).

METHODS

Human monocytes purified by CD14+ immuno-magnetic beads were differentiated and induced into immature DCs, which were randomly divided into 6 groups, Group A treated with PBS, Group B treated with OX-LDL, Group C and D treated respectively with GRb1 and ciglitazone, Group E and F were pretreated with the two testing drugs respectively followed by OX-LDL. The immuno-phenotypic expression (CD40, CD1a, and HLA-DR) and endocytosis function of DCs were examined using flow cytometry, the concentration of interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-alpha) in the culture supernatants were measured with ELISA.

RESULTS

Compared with Group B, Group E showed significantly lowered immuno-phenotypic expression of DCs in terms of CD40 (67.4 +/- 1.62 vs. 145.69 +/- 14.86), CD1a (79.64 +/- 3.04 vs. 159.89 +/- 6.09), and HLA-DR (46.43 +/- 2.85 vs. 99.33 +/- 17.11), as well as higher endocytosis level (88.13% +/- 1.06% vs. 25.90% +/- 5.77%, all P < 0.01). Meantime, the serum levels of IL-12 (88.65 +/- 5.59 ng/L vs. 716.69 +/- 36.35 ng/L) and TNF-alpha (133.27 +/- 11.98 ng/L vs. 968.10 +/- 36.42 ng/L) obviously decreased (P < 0.01). The surface molecular expression of DCs and the secretion of inflammatory factors in Group F also obviously decreased, showing insignificant difference from Group E (P > 0.05).

CONCLUSION

GRb1 could obviously inhibit the OX-LDL-induced maturation of DCs, showing similar effects to ciglitazone.

摘要

目的

探讨人参皂苷Rb1（GRb1）对氧化型低密度脂蛋白（OX-LDL）诱导的单核细胞来源树突状细胞（DCs）免疫成熟的影响。

方法

采用CD14+免疫磁珠法纯化人单核细胞，诱导分化为未成熟DCs，随机分为6组，A组用PBS处理，B组用OX-LDL处理，C组和D组分别用GRb1和吡格列酮处理，E组和F组分别先用两种受试药物预处理后再用OX-LDL处理。采用流式细胞术检测DCs的免疫表型表达（CD40、CD1a和HLA-DR）及内吞功能，用ELISA法检测培养上清液中白细胞介素-12（IL-12）和肿瘤坏死因子α（TNF-α）的浓度。

结果

与B组相比，E组DCs的免疫表型表达CD40（67.4±1.62对145.69±14.86）、CD1a（79.64±3.04对159.89±6.09）和HLA-DR（46.43±2.85对99.33±17.11）显著降低，内吞水平升高（88.13%±1.06%对25.90%±5.77%，均P<0.01）。同时，IL-12（88.65±5.59 ng/L对716.69±36.35 ng/L）和TNF-α（133.27±11.98 ng/L对968.10±36.42 ng/L）的血清水平明显降低（P<0.01）。F组DCs的表面分子表达和炎症因子分泌也明显降低，与E组相比差异无统计学意义（P>0.05）。