Liu Hong-ying, Shi Da-zhuo, Ge Jun-bo
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Mar;31(3):350-4.
To examine the effect of ginsenoside Rb1 (GRb1) on the immune maturation of monocyte-derived dendritic cells (DCs) induced by oxidized low-density lipoprotein (OX-LDL).
Human monocytes purified by CD14+ immuno-magnetic beads were differentiated and induced into immature DCs, which were randomly divided into 6 groups, Group A treated with PBS, Group B treated with OX-LDL, Group C and D treated respectively with GRb1 and ciglitazone, Group E and F were pretreated with the two testing drugs respectively followed by OX-LDL. The immuno-phenotypic expression (CD40, CD1a, and HLA-DR) and endocytosis function of DCs were examined using flow cytometry, the concentration of interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-alpha) in the culture supernatants were measured with ELISA.
Compared with Group B, Group E showed significantly lowered immuno-phenotypic expression of DCs in terms of CD40 (67.4 +/- 1.62 vs. 145.69 +/- 14.86), CD1a (79.64 +/- 3.04 vs. 159.89 +/- 6.09), and HLA-DR (46.43 +/- 2.85 vs. 99.33 +/- 17.11), as well as higher endocytosis level (88.13% +/- 1.06% vs. 25.90% +/- 5.77%, all P < 0.01). Meantime, the serum levels of IL-12 (88.65 +/- 5.59 ng/L vs. 716.69 +/- 36.35 ng/L) and TNF-alpha (133.27 +/- 11.98 ng/L vs. 968.10 +/- 36.42 ng/L) obviously decreased (P < 0.01). The surface molecular expression of DCs and the secretion of inflammatory factors in Group F also obviously decreased, showing insignificant difference from Group E (P > 0.05).
GRb1 could obviously inhibit the OX-LDL-induced maturation of DCs, showing similar effects to ciglitazone.
探讨人参皂苷Rb1（GRb1）对氧化型低密度脂蛋白（OX-LDL）诱导的单核细胞来源树突状细胞（DCs）免疫成熟的影响。
采用CD14+免疫磁珠法纯化人单核细胞，诱导分化为未成熟DCs，随机分为6组，A组用PBS处理，B组用OX-LDL处理，C组和D组分别用GRb1和吡格列酮处理，E组和F组分别先用两种受试药物预处理后再用OX-LDL处理。采用流式细胞术检测DCs的免疫表型表达（CD40、CD1a和HLA-DR）及内吞功能，用ELISA法检测培养上清液中白细胞介素-12（IL-12）和肿瘤坏死因子α（TNF-α）的浓度。
与B组相比，E组DCs的免疫表型表达CD40（67.4±1.62对145.69±14.86）、CD1a（79.64±3.04对159.89±6.09）和HLA-DR（46.43±2.85对99.33±17.11）显著降低，内吞水平升高（88.13%±1.06%对25.90%±5.77%，均P<0.01）。同时，IL-12（88.65±5.59 ng/L对716.69±36.35 ng/L）和TNF-α（133.27±11.98 ng/L对968.10±36.42 ng/L）的血清水平明显降低（P<0.01）。F组DCs的表面分子表达和炎症因子分泌也明显降低，与E组相比差异无统计学意义（P>0.05）。
GRb1能明显抑制OX-LDL诱导的DCs成熟，与吡格列酮作用相似。
