Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China.
J Cardiovasc Pharmacol. 2010 Aug;56(2):177-83. doi: 10.1097/FJC.0b013e3181e5f0f8.
TongXinLuo (TXL) is a traditional Chinese herbal medicine with multiple vasoprotective activities. Dendritic cells (DCs) play an active role in the immunological processes related to atherosclerosis. The purpose of this study was to determine the effect and possible mechanisms of TXL on oxidized low-density lipoprotein (OX-LDL)-induced maturation and immune function of DCs. Human monocyte-derived DCs were incubated with TXL or ciglitazone and were subsequently stimulated with OX-LDL to induce maturation. Similar to ciglitazone, a peroxisome proliferator-activated receptor (PPAR) gamma agonist, TXL could significantly reduce the maturation-associated markers induced by OX-LDL, such as CD40, CD86, CD1a, and human leukocyte antigen-DR; improved the endocytotic function; and decreased secretions of cytokine interleukin-12 and tumor necrosis factor alpha. These inhibitory effects of TXL could be partly reversed by silencing the expression of PPAR gamma in DCs. In conclusion, TXL could inhibit OX-LDL-induced maturation of DCs through activating PPAR gamma pathway.
通心络（TXL）是一种具有多种血管保护作用的中药。树突状细胞（DCs）在与动脉粥样硬化相关的免疫过程中发挥着积极的作用。本研究旨在确定 TXL 对氧化型低密度脂蛋白（OX-LDL）诱导的 DC 成熟和免疫功能的影响及其可能的机制。将人单核细胞来源的 DC 与 TXL 或吡格列酮孵育，然后用 OX-LDL 刺激以诱导成熟。与过氧化物酶体增殖物激活受体（PPAR）γ激动剂吡格列酮类似，TXL 可显著降低 OX-LDL 诱导的成熟相关标志物，如 CD40、CD86、CD1a 和人类白细胞抗原-DR；改善内吞作用；并减少细胞因子白细胞介素-12 和肿瘤坏死因子-α的分泌。这些抑制作用可部分通过沉默 DC 中 PPARγ的表达来逆转。总之，TXL 可通过激活 PPARγ通路抑制 OX-LDL 诱导的 DC 成熟。
