De Somer F, Van Landschoot A, Van Nooten G, Delanghe J
Heart Centre, University Hospital Ghent, Belgium.
Perfusion. 2008 Jul;23(4):215-21. doi: 10.1177/0267659108099814.
Protein adsorption onto polymers remains a problem. In recent years, several protein-repellent PVC tubings have been developed. Although several studies report the interaction between plasma coagulation proteins and PVC, few address the interaction with other plasma proteins. Two commercial brands of untreated medical grade PVC tubing, phosphorylcholine-coated PVC tubing, triblock-copolymer (polycaprolactone-polydimethylsiloxane-polycaprolactone)-treated PVC tubing and poly-2-methoxyethylacrylate (PMEA)-coated tubing were exposed for 60 minutes to human plasma. A broad spectrum of plasma proteins was found on all tubing. The adsorbed albumin to total protein ratio is lower than the similar ratio in plasma while alpha1 and alpha2 globulins are over-represented in the protein spectrum. On PMEA tubing, not only alpha globulins, but also beta and gamma globulins, are found in high concentrations in the adsorbed protein. PMEA tubing and uncoated PVC tubing of brand B had a higher amount of protein adsorbed compared against all other tubing (p < 0.05). There were no statistical differences in protein adsorption between the triblock-copolymer-treated tubing, the phosphorylcholine-coated tubing and the uncoated PVC tubing of brand A. The average thickness of the protein layer was 23 nm. Plasma protein adsorption still exists on uncoated and protein-repellent tubing and can initiate a systemic inflammatory reaction.
蛋白质在聚合物上的吸附仍然是一个问题。近年来，已经开发出了几种抗蛋白质吸附的聚氯乙烯（PVC）管材。尽管有几项研究报告了血浆凝血蛋白与PVC之间的相互作用，但很少有研究涉及与其他血浆蛋白的相互作用。将两个未处理的医用级PVC管材商业品牌、磷酸胆碱涂层PVC管材、三嵌段共聚物（聚己内酯-聚二甲基硅氧烷-聚己内酯）处理的PVC管材和聚-2-甲氧基乙基丙烯酸酯（PMEA）涂层管材暴露于人体血浆中60分钟。在所有管材上都发现了种类广泛的血浆蛋白。吸附的白蛋白与总蛋白的比例低于血浆中的类似比例，而α1和α2球蛋白在蛋白质谱中所占比例过高。在PMEA管材上，不仅α球蛋白，而且β和γ球蛋白在吸附的蛋白质中浓度也很高。与所有其他管材相比，品牌B的PMEA管材和未涂层PVC管材吸附的蛋白质量更高（p < 0.05）。三嵌段共聚物处理的管材、磷酸胆碱涂层管材和品牌A的未涂层PVC管材之间在蛋白质吸附方面没有统计学差异。蛋白质层的平均厚度为23纳米。未涂层和抗蛋白质吸附的管材上仍然存在血浆蛋白吸附，并且可能引发全身炎症反应。
