Li Lin, Liang Chao-zhao, Zhang Xian-sheng, Hao Zong-yao, Fan Song, Gu Yuan
Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.
Zhonghua Nan Ke Xue. 2011 Mar;17(3):224-8.
To investigate the difference in intracellular calcium ion [Ca2+]i concentration in prostate smooth muscle cells (PSMCs) between SD rat models of chronic abacterial prostatitis (CAP) and normal controls, and to evaluate the role of [Ca2+]i concentration in CAP.
We established CAP models in SD rats using purified prostate protein and Freund's complete adjuvant, cultured in vitro and then purified the PSMCs of both the CAP models and normal controls. Continuous dynamic scanning was performed under the laser confocal scanning microscope after incubation of the cells with FLUO-3AM.
The fluorescence intensities of [Ca2+]i in the PSMCs were 80.39 +/- 9.00 and 27.95 +/- 10.04 in the CAP models and normal controls, respectively, with statistically significant differences between the two groups (P < 0.01).
The concentration of [Ca2+]i increased in the PSMCs of the CAP rat models, which might enhance the constriction of PSMCs and subsequently increase the sensibility to pain and cause lower abdominal pain.
探讨慢性非细菌性前列腺炎（CAP）SD大鼠模型与正常对照大鼠前列腺平滑肌细胞（PSMCs）内钙离子[Ca2+]i浓度的差异，评估[Ca2+]i浓度在CAP中的作用。
用纯化的前列腺蛋白和弗氏完全佐剂建立SD大鼠CAP模型，体外培养后纯化CAP模型组和正常对照组的PSMCs。细胞用FLUO-3AM孵育后，在激光共聚焦扫描显微镜下进行连续动态扫描。
CAP模型组和正常对照组PSMCs中[Ca2+]i的荧光强度分别为80.39±9.00和27.95±10.04，两组间差异有统计学意义（P<0.01）。
CAP大鼠模型的PSMCs中[Ca2+]i浓度升高，这可能增强PSMCs的收缩，进而增加对疼痛的敏感性并导致下腹部疼痛。
