Nanomaterial Toxicology Group, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M. G. Marg, Lucknow 226001, India.
J Biomed Nanotechnol. 2011 Feb;7(1):177-8. doi: 10.1166/jbn.2011.1257.
C60-fullerene has promising biological applications, such as drug delivery, biosensors, diagnosis and theraupetics. Despite of these applications, several in vitro studies have also reported the DNA damaging potential of this nanomaterial. Though, very little is known about the mechanism involved behind the fullerene mediated DNA damage. Our study was aimed at identifying the binding site of fullerene in the ATP binding domain of human topoisomerase II alpha, a major enzyme involved in maintaining DNA topology. In silico studies of fullerene with the enzyme demonstrated that it can interact with the active site residues of this enzyme through hydrophobic, pi-stacking and van der Waals interactions and could inhibit the activity of this enzyme.
C60 富勒烯在药物输送、生物传感器、诊断和治疗等方面具有广阔的生物应用前景。尽管有这些应用，但几项体外研究也报告了这种纳米材料的 DNA 损伤潜力。然而，对于富勒烯介导的 DNA 损伤背后涉及的机制知之甚少。我们的研究旨在确定富勒烯在参与维持 DNA 拓扑结构的主要酶——人拓扑异构酶 IIα的 ATP 结合域中的结合位点。富勒烯与该酶的计算机模拟研究表明，它可以通过疏水、π-堆积和范德华相互作用与该酶的活性位点残基相互作用，并可能抑制该酶的活性。
