Shanghai Key Laboratory of Functional Materials Chemistry & Department of Chemistry, East China University of Science and Technology, Shanghai, 200237, PR China.
Environ Sci Technol. 2011 May 1;45(9):4046-52. doi: 10.1021/es104155r. Epub 2011 Apr 12.
A novel facile method for on-site detection of substituted aromatic pollutants in water using thin layer chromatography (TLC) combined with surface-enhanced Raman spectroscopy (SERS) was explored. Various substituted aromatics in polluted water were separated by a convenient TLC protocol and then detected using a portable Raman spectrometer with the prepared silver colloids serving as SERS-active substrates. The effects of operating conditions on detection efficacy were evaluated, and the application of TLC-SERS to on-site detection of artificial and real-life samples of aromatics/polluted water was systematically investigated. It was shown that commercially available Si 60-F(254) TLC plates were suitable for separation and displayed low SERS background and good separation efficiency, 2 mM silver colloids, 20 mM NaCl (working as aggregating agent), 40 mW laser power, and 50 s intergration time were appropriate for the detection regime. Furthermore, qualitative and quantitative detection of most of substituted aromatic pollutants was found to be readily accomplished using the developed TLC-SERS technique, which compared well with GC-MS in terms of identification ability and detection accuracy, and a limit of detection (LOD) less than 0.2 ppm (even at ppb level for some analytes) could be achieved under optimal conditions. The results reveal that the presented convenient method could be used for the effective separation and detection of the substituted aromatic pollutants of water on site, thus reducing possible influences of sample transportation and contamination while shortening the overall analysis time for emergency and routine monitoring of the substituted aromatics/polluted water.
一种使用薄层色谱（TLC）结合表面增强拉曼光谱（SERS）现场检测水中取代芳烃污染物的新方法被探索。通过方便的 TLC 方案分离受污染水中的各种取代芳烃，然后使用带有制备的银胶体作为 SERS 活性衬底的便携式拉曼光谱仪进行检测。评估了操作条件对检测效果的影响，并系统地研究了 TLC-SERS 在人工和真实生活水样中芳烃/污染水的现场检测中的应用。结果表明，市售的 Si 60-F(254) TLC 板适用于分离，并且显示出低 SERS 背景和良好的分离效率，2 mM 银胶体、20 mM NaCl（用作聚集剂）、40 mW 激光功率和 50 s 积分时间适用于检测方案。此外，使用开发的 TLC-SERS 技术可以轻松完成对大多数取代芳烃污染物的定性和定量检测，与 GC-MS 相比，在识别能力和检测精度方面表现良好，在最佳条件下可以达到低于 0.2 ppm（对于某些分析物甚至达到 ppb 水平）的检测限（LOD）。结果表明，所提出的简便方法可用于现场有效分离和检测水中的取代芳烃污染物，从而减少了样品运输和污染的可能影响，同时缩短了对取代芳烃/污染水进行紧急和常规监测的整体分析时间。
