Department of Periodontics, Louisiana State University Health Science Center, School of Dentistry, New Orleans, LA 70119, USA.
J Periodontol. 2012 Jan;83(1):90-100. doi: 10.1902/jop.2011.100733. Epub 2011 Apr 12.
Titanium implants are widely used in dentistry to replace lost teeth. Various surface modifications have been used to improve implant retention and osseointegration. This study is designed to compare the ability of three titanium surfaces to promote cell attachment and cell motility of cells relevant to periodontal tissues.
Three clinically relevant surfaces were tested: 1) machined titanium; 2) a titanium surface roughened through acid etching (dual thermal-etched titanium [DTET]); and 3) a titanium surface roughened with nanometer-scale calcium phosphate deposition (nanoscale calcium phosphate-impregnated titanium [NCPIT]). Cell attachment and migration were examined for four cell types: rat osteosarcoma cells, human osteoblasts, and gingival and periodontal ligament (PDL) fibroblasts.
All four cell types attached to each of the three titanium surfaces equally by 2 hours, and the PDL and gingival fibroblasts generally displayed less attachment than the osteosarcoma cells and osteoblasts. The cells displayed differential motility and long-term attachment to each of the titanium surfaces. Osteosarcoma cells displayed preferential motility on NCPIT, whereas PDL fibroblasts were more motile on machined titanium, and gingival fibroblasts moved more rapidly on both DTET and NCPIT. Osteoblasts displayed little motility on any of the titanium surfaces and lost viability on NCPIT after 24 hours. Gingival fibroblasts lost attachment to machined titanium.
Periodontal cells displayed differential motility and long-term attachment to titanium surfaces. Selective modification of titanium surface properties in various regions of an implant may be useful in guiding specific cell populations to specific locations where they might best aid in osseointegration and soft tissue remodeling.
钛植入物在牙科中被广泛用于替代缺失的牙齿。已经使用了各种表面改性方法来提高植入物的保留率和骨整合。本研究旨在比较三种钛表面促进与牙周组织相关的细胞附着和细胞迁移的能力。
测试了三种临床相关的表面：1）机械加工的钛；2）通过酸蚀处理（双热蚀刻钛[DTET]）粗糙化的钛表面；3）用纳米级磷酸钙沉积粗糙化的钛表面（纳米级磷酸钙浸渍钛[NCPIT]）。研究了四种细胞类型的细胞附着和迁移：大鼠骨肉瘤细胞、人成骨细胞以及牙龈和牙周韧带（PDL）成纤维细胞。
所有四种细胞类型在 2 小时内都能同等地附着在三种钛表面上，PDL 和牙龈成纤维细胞的附着通常比骨肉瘤细胞和成骨细胞少。这些细胞对每种钛表面表现出不同的迁移能力和长期附着能力。骨肉瘤细胞在 NCPIT 上显示出优先迁移，而 PDL 成纤维细胞在机械加工的钛上更具迁移性，牙龈成纤维细胞在 DTET 和 NCPIT 上迁移得更快。成骨细胞在任何钛表面上都表现出很少的迁移能力，并且在 24 小时后在 NCPIT 上失去活力。牙龈成纤维细胞失去了对机械加工钛的附着。
牙周细胞对钛表面表现出不同的迁移能力和长期附着能力。在植入物的各个区域选择性地修饰钛表面特性可能有助于引导特定细胞群体到达最佳的骨整合和软组织重塑位置。
