Ramaglia Luca, Di Spigna Gaetano, Capece Gabriele, Sbordone Carolina, Salzano Salvatore, Postiglione Loredana
Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples "Federico II", Naples, Italy.
Department of Translational Medical Sciences, University of Naples "Federico II", Naples, Italy.
Clin Oral Investig. 2015 Dec;19(9):2245-53. doi: 10.1007/s00784-015-1469-5. Epub 2015 Apr 17.
Analysis of the effects of titanium surface properties on the biological behavior of human gingival fibroblasts (HGFs).
HGFs were in vitro cultured on a titanium surface modified by a dual acid-etched procedure and on a control machined surface. Cell adhesion, proliferation, apoptosis, production of certain extracellular matrix (ECM) proteins, and expression of granulocyte macrophage-colony stimulating factor receptor (GM-CSFR) were investigated using in each experiment a total of 18 samples for each titanium surface.
Cell attachment at 3 h of culture was statistically significantly higher on the etched surface. HGF growth increased on both surfaces during the entire experimental period and at day 14 of culture cell proliferation was statistically significantly higher on the treated surface than on the control. No statistically significant differences in percentage of apoptosis events were observed between the surfaces. ECM protein production increased progressively over time on both surfaces. A statistically significant deposition was observed at day 7 and 14 for collagen I and only at day 14 for fibronectin and tenascin, when compared to the baseline. GM-CSFR registered a positive expression on both surfaces, statistically significant at day 14 on the etched surface in comparison with the machined one.
Data showed that titanium surface microtopography modulates in vitro cell response and phenotypical expression of HGFs. The etched surface promoted a higher cell proliferation and differentiation improving the biological behavior of HGFs.
Results suggest a possible beneficial effect of surface etching modification on peri-implant biological integration and soft tissue healing which is critical for the formation of a biological seal around the neck of dental implants.
分析钛表面特性对人牙龈成纤维细胞（HGFs）生物学行为的影响。
将HGFs在经双重酸蚀处理改性的钛表面和对照机械加工表面上进行体外培养。在每个实验中，对每种钛表面共使用18个样本，研究细胞黏附、增殖、凋亡、某些细胞外基质（ECM）蛋白的产生以及粒细胞巨噬细胞集落刺激因子受体（GM-CSFR）的表达。
培养3小时时，蚀刻表面的细胞附着在统计学上显著更高。在整个实验期间，两个表面上的HGF生长均增加，并且在培养第14天时，处理表面上的细胞增殖在统计学上显著高于对照表面。两个表面之间未观察到凋亡事件百分比的统计学显著差异。两个表面上的ECM蛋白产生均随时间逐渐增加。与基线相比，在第7天和第14天观察到I型胶原的统计学显著沉积，而纤连蛋白和腱生蛋白仅在第14天有显著沉积。GM-CSFR在两个表面上均呈阳性表达，与机械加工表面相比，在蚀刻表面上第14天时具有统计学显著性。
数据表明钛表面微观形貌可调节体外细胞反应和HGFs的表型表达。蚀刻表面促进了更高的细胞增殖和分化，改善了HGFs的生物学行为。
结果表明表面蚀刻改性对种植体周围生物整合和软组织愈合可能具有有益作用，这对于在牙种植体颈部形成生物密封至关重要。
