Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
Institute of Quantum Optics, Leibniz University of Hannover, Welfengarten 1, 30167 Hannover, Germany.
Int J Mol Sci. 2020 Nov 10;21(22):8442. doi: 10.3390/ijms21228442.
Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell-implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.
细胞相容性对于植入物的批准至关重要。然而，最初的体外筛选主要包括黏附性永生化细胞的数量和细胞毒性。其他重要参数，如细胞迁移以及对天然组织细胞与植入物表面相互作用的深入了解，很少被考虑。我们使用成纤维细胞和骨细胞的原代和永生化细胞系研究了不同的激光制造的尖峰结构，并对细胞面积、纵横比和焦点粘连进行了定量分析。此外，我们研究了与尖峰形貌的三维细胞相互作用，并开发了一种针对长期监测不透明材料的定制迁移检测方法。虽然小尖峰上的成纤维细胞和骨细胞保持正常形态，但中大和大尖峰上的细胞会陷入结构中，从而影响细胞骨架的组成，进而改变细胞形状。在 14 天内，小尖峰上的迁移似乎更强，这可能是由于适当的焦点粘连形成和完整的细胞骨架所致，而与永生化细胞系相比，人原代细胞表现出差异。使用原代细胞、分析细胞-植入物结构相互作用以及细胞迁移可能会增强对细胞相容性的评估，从而提高植入材料潜在体内性能的有效性。
