School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
Tissue Eng Part C Methods. 2011 Sep;17(9):887-94. doi: 10.1089/ten.TEC.2011.0098. Epub 2011 May 25.
The function of an implanted tissue-engineered pancreatic construct is influenced by many in vivo factors; however, assessing its function is based primarily on end physiologic effects. As oxygen significantly affects cell function, we established a dual perfluorocarbon method that utilizes (19)F nuclear magnetic resonance spectroscopy, with perfluorocarbons as oxygen concentration markers, to noninvasively monitor dissolved oxygen concentration (DO) in βTC-tet cell-containing alginate beads and at the implantation milieu. Beads were implanted in the peritoneal cavity of normal and streptozotocin-induced diabetic mice. Using this method, the feasibility of acquiring real-time in vivo DO measurements was demonstrated. Results showed that the mouse peritoneal environment is hypoxic and the DO is further reduced when βTC-tet cell constructs were implanted. The DO within cell-containing beads decreased considerably over time and could be correlated with the relative changes in the number of viable encapsulated cells. The reduction of construct DO due to the metabolic activity of the βTC-tet cells was also compatible with the implant therapeutic function, as observed in the reversal of hyperglycemia in diabetic mice. The importance of these findings in assessing implant functionality and host animal physiology is discussed.
植入的组织工程胰腺构建体的功能受到许多体内因素的影响；然而，其功能的评估主要基于终末生理效应。由于氧气对细胞功能有显著影响，我们建立了一种双重全氟化碳方法，利用（19）氟核磁共振波谱，以全氟化碳作为氧浓度标记物，非侵入性地监测含βTC-tet 细胞的藻酸盐珠和植入环境中的溶解氧浓度（DO）。将珠子植入正常和链脲佐菌素诱导的糖尿病小鼠的腹腔中。使用这种方法，证明了实时体内 DO 测量的可行性。结果表明，小鼠腹膜环境是缺氧的，当βTC-tet 细胞构建体被植入时，DO 进一步降低。含细胞珠内的 DO 随时间的推移显著下降，并且可以与包封细胞的相对数量变化相关联。由于βTC-tet 细胞的代谢活性导致的构建体 DO 的减少也与植入治疗功能兼容，如在糖尿病小鼠中观察到的高血糖逆转。讨论了这些发现对评估植入物功能和宿主动物生理学的重要性。
