Kristoffersen E K, Ulvestad E, Vedeler C A, Matre R
Broegelmann Research Laboratory for Microbiology, Gade Institute, University of Bergen, Norway.
Scand J Immunol. 1990 Nov;32(5):561-4. doi: 10.1111/j.1365-3083.1990.tb03197.x.
Fc receptors for IgG, FcRI (CD64), FcRII (CD32), and FcRIII (CD16) in human placenta were studied by indirect immunohistochemistry using avidin-biotin peroxidase complexes for the staining of cryostat sections. The MoAb 32.2 against FcRI stained cells in the loose connective tissue of the placental villi. The MoAb IV3 (FcRII) and C1KM5 (FcRII) also stained stromal cells and in addition stained the endothelium of the placental villi. The MoAb anti-Leu-11b against FcRIII and B1D6 against a 40-kDa FcR from placenta stained both stromal cells and endothelium as well as the fetal trophoblasts lining the villi. The MoAb 3G8 (FcRIII) also stained trophoblasts and stromal cells but did not stain the endothelium. The heterogeneity of FcR expression on human placenta is established. The function of the different receptors is still unclear.
采用抗生物素蛋白-生物素过氧化物酶复合物对恒冷箱切片进行染色，通过间接免疫组织化学方法研究了人胎盘中IgG的Fc受体、FcRI（CD64）、FcRII（CD32）和FcRIII（CD16）。抗FcRI的单克隆抗体32.2对胎盘绒毛疏松结缔组织中的细胞进行了染色。抗FcRII的单克隆抗体IV3和C1KM5也对基质细胞进行了染色，此外还对胎盘绒毛的内皮细胞进行了染色。抗FcRIII的单克隆抗体抗-Leu-11b和抗胎盘40 kDa FcR的B1D6对基质细胞、内皮细胞以及绒毛内衬的胎儿滋养层细胞均进行了染色。单克隆抗体3G8（FcRIII）也对滋养层细胞和基质细胞进行了染色，但未对内皮细胞进行染色。人胎盘上FcR表达的异质性得以确立。不同受体的功能仍不清楚。
