Vedeler C A, Matre R, Kristoffersen E K, Ulvestad E
Broegelmann Research Laboratory for Microbiology, University of Bergen, Norway.
Acta Neurol Scand. 1991 Sep;84(3):177-80. doi: 10.1111/j.1600-0404.1991.tb04933.x.
The presence of Fc-receptors for IgG, FcRI (CD64), FcRII (CD32) and FcRIII (CD16) in human peripheral nerves was studied by indirect immunofluorescence staining of cryostat sections with monoclonal antibodies (mabs). The mabs 32.2 against FcRI, IV.3 and C1KM5 against FcRII, B1D6 against a 40 kD FcR with low affinity for IgG, and Leu-11b and 3G8 against FcRIII stained a few scattered cells in the endoneurium, apparently monocytes/macrophages. In addition, B1D6 and Leu11b gave a membranous staining of Schwann cells, endothelial cells and perineurial cells. Axons and fibroblasts were not stained by any of the mabs. The FcR may be important in binding immune complexes, in phagocytosis, in the release of lymphokines as well as cytotoxic and inflammatory mediators and in local immunoregulation.
采用单克隆抗体对冰冻切片进行间接免疫荧光染色，研究了人外周神经中IgG的Fc受体FcRI（CD64）、FcRII（CD32）和FcRIII（CD16）的存在情况。抗FcRI的单克隆抗体32.2、抗FcRII的IV.3和C1KM5、抗与IgG亲和力低的40kD FcR的B1D6以及抗FcRIII的Leu-11b和3G8，对神经内膜中一些散在细胞进行了染色，这些细胞明显为单核细胞/巨噬细胞。此外，B1D6和Leu11b对雪旺细胞、内皮细胞和神经束膜细胞进行了膜染色。轴突和成纤维细胞未被任何单克隆抗体染色。Fc受体在结合免疫复合物、吞噬作用、淋巴因子以及细胞毒性和炎症介质的释放以及局部免疫调节中可能起重要作用。
