从孕妇淋巴细胞培养上清液中分离纯化一种早期妊娠因子样分子：II. 该分子鉴定为Fc受体样分子：初步报告。

Isolation and purification of an early pregnancy factor-like molecule from culture supernatants obtained from lymphocytes of pregnant women: II. Identification of the molecule as a Fc-receptor-like molecule: a preliminary report.

作者信息

Aranha C, Bordekar A, Shahani S

机构信息

Institute for Research in Reproduction (ICMR), Mumbai, India.

出版信息

J Assist Reprod Genet. 1998 Nov;15(10):619-24. doi: 10.1023/a:1020389512677.

DOI:10.1023/a:1020389512677

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3454852/

Abstract

PURPOSE

Early pregnancy factor (EPF)-like activity from culture supernatants obtained from stimulated lymphocytes of pregnant women was characterized and identified.

METHODS

The enzyme-linked immunosorbent assay depending on the presence of "Fc" receptors on bovine spermatozoa was used to identify the EPF-like molecule purified by gel filtration and reverse-phase high-performance liquid chromatography.

RESULTS

The results indicated that the crude lymphocyte culture supernatant, the EPF-positive G IV fraction obtained on gel filtration, and the EPF-positive reverse-phase high-performance liquid chromatography protein readily bound with the different concentrations of aggregated human gamma-globulin in a manner similar to that in which the standard control of aggregated human gamma-globulin binds to the bovine spermatozoa.

CONCLUSIONS

EPF-like activity synthesized and secreted by lymphocytes during pregnancy may be a Fc-receptor-like molecule.

摘要

目的

对从孕妇刺激淋巴细胞获得的培养上清液中的早期妊娠因子（EPF）样活性进行表征和鉴定。

方法

采用基于牛精子上“Fc”受体存在的酶联免疫吸附测定法，鉴定通过凝胶过滤和反相高效液相色谱法纯化的EPF样分子。

结果

结果表明，粗淋巴细胞培养上清液、凝胶过滤获得的EPF阳性G IV级分以及EPF阳性反相高效液相色谱蛋白，能以类似于人聚合γ球蛋白标准对照与牛精子结合的方式，与不同浓度的人聚合γ球蛋白结合。

结论

妊娠期间淋巴细胞合成和分泌的EPF样活性可能是一种Fc受体样分子。

相似文献

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Isolation and purification of an early pregnancy factor-like molecule from culture supernatants obtained from lymphocytes of pregnant women: II. Identification of the molecule as a Fc-receptor-like molecule: a preliminary report.从孕妇淋巴细胞培养上清液中分离纯化一种早期妊娠因子样分子：II. 该分子鉴定为Fc受体样分子：初步报告。

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Isolation and purification of an early pregnancy factor-like molecule from culture supernatants obtained from lymphocytes of pregnant women.从孕妇淋巴细胞培养上清液中分离纯化一种早期妊娠因子样分子。

J Assist Reprod Genet. 1998 Mar;15(3):117-24. doi: 10.1023/a:1023052703640.

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The purification of early-pregnancy factor to homogeneity from human platelets and identification as chaperonin 10.从人血小板中纯化出均一的早孕因子并鉴定其为伴侣蛋白10。

Eur J Biochem. 1994 Jun 1;222(2):551-60. doi: 10.1111/j.1432-1033.1994.tb18897.x.

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